文献类型：期刊文献

英文题名：Revealing the roles of GORK channels and NADPH oxidase in acclimation to hypoxia in Arabidopsis

作者：Wang, Feifei Chen, Zhong-Hua Liu, Xiaohui Colmer, Timothy D. Shabala, Lana Salih, Anya Zhou, Meixue Shabala, Sergey

第一作者：Wang, Feifei

通信作者：Shabala, S[1]

机构：[1]Univ Tasmania, Sch Land & Food, Hobart, Tas 7001, Australia;[2]Western Sydney Univ, Sch Sci & Hlth, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia;[3]Guizhou Inst Technol, Sch Light Ind Engn, Guiyang 550003, Guizhou, Peoples R China;[4]Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia;[5]Univ Western Australia, Inst Agr, Crawley, WA 6009, Australia

第一机构：Univ Tasmania, Sch Land & Food, Hobart, Tas 7001, Australia

通信机构：corresponding author), Univ Tasmania, Sch Land & Food, Hobart, Tas 7001, Australia.

年份：2017

卷号：68

期号：12

起止页码：3191-3204

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;Scopus(收录号：2-s2.0-85031817948);WOS:【SCI-EXPANDED(收录号:WOS:000407488500014)】；

基金：This project is supported by an Australian Research Council (ARC) Discovery Project to TDC and SS. Z-HC is supported by an ARC Discovery Early Career Researcher Award (DE140101143) and a 1000-Plan project, China. XL is the receipt of a China Scholarship Council award. We thank Dr Rong Liu, Linda Westmoreland, Liz Kabonoff, Dr Sumedha Dharmaratne, Rosemary Freeman, Honghong Wu, and Xin Huang for their technical assistance. Our special thanks go to the Western Sydney University Confocal Bio-Imaging Facility.

语种：英文

外文关键词：Calcium; epidermis; fluorescence dyes; H2O2; homeostasis; potassium; reactive oxygen species; signalling; stele; superoxide

摘要：Regulation of root cell K+ is essential for acclimation to low oxygen stress. The potential roles of GORK (depolarization-activated guard cell outward-rectifying potassium) channels and RBOHD (respiratory burst oxidase homologue D) in plant adaptive responses to hypoxia were investigated in the context of tissue specificity (epidermis versus stele; elongation versus mature zone) in roots of Arabidopsis. The expression of GORK and RBOHD was down-regulated by 2- to 3-fold within 1 h and 24 h of hypoxia treatment in Arabidopsis wild-type (WT) roots. Interestingly, a loss of the functional GORK channel resulted in a waterlogging-tolerant phenotype, while rbohD knockout was sensitive to waterlogging. To understand their functions under hypoxia stress, we studied K+, Ca2+, and reactive oxygen species (ROS) distribution in various root cell types. gork1-1 plants had better K+ retention ability in both the elongation and mature zone compared with the WT and rbohD under hypoxia. Hypoxia induced a Ca2+ increase in each cell type after 72 h, and the increase was much less pronounced in rbohD than in the WT. In most tissues except the elongation zone in rbohD, the H2O2 concentration had decreased after 1 h of hypoxia, but then increased significantly after 24 h of hypoxia in each zone and tissue, further suggesting that RBOHD may shape hypoxia-specific Ca2+ signatures via the modulation of apoplastic H2O2 production. Taken together, our data suggest that plants lacking functional GORK channels are more capable of retaining K+ for their better performance under hypoxia, and that RBOHD is crucial in hypoxia-induced Ca2+ signalling for stress sensing and acclimation mechanism.