文献类型：期刊文献

英文题名：Community structure and metabolic potentials of keystone taxa and their associated bacteriophages within rice root endophytic microbiome in response to metal(loid)s contamination

作者：Jia, Yanlong Huang, Duanyi Lan, Xiaolong Sun, Xiaoxu Lin, Wenjie Sun, Weimin Wang, Yize

第一作者：贾彦龙;Jia, Yanlong

通信作者：Wang, YZ[1]

机构：[1]Hanshan Normal Univ, Sch Chem & Environm Engn, Chaozhou 521041, Peoples R China;[2]Guizhou Inst Technol, Sch Resources & Environm Engn, Guiyang 550002, Peoples R China;[3]Guangdong Acad Sci, Inst Ecoenvironm & Soil Sci, Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangdong Key Lab Integrated Agroenvironm Pollut C, Guangzhou 510650, Peoples R China;[4]Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Peoples R China

第一机构：Hanshan Normal Univ, Sch Chem & Environm Engn, Chaozhou 521041, Peoples R China

通信机构：corresponding author), 808 Tianyuan Rd, Guangzhou, Guangdong, Peoples R China.

年份：2025

卷号：372

外文期刊名：ENVIRONMENTAL POLLUTION

收录：;EI(收录号：20251118047387);Scopus(收录号：2-s2.0-86000575247);WOS:【SCI-EXPANDED(收录号:WOS:001448056900001)】；

基金：This work was funded by the multiple funding agencies, including the Guizhou Provincial Key Technology R & D Program (Project No. [2021]492) , National Natural Science Foundation of China (Projects No. 41967041, 42307345, 42307296) , Project of Educational Commission of Guangdong Province of China (Projects No. 2023KSYS007, 2023KTSCX078, 2023KCXTD023, 2020ZDZX1032) , GDAS' Project of Science and Technology Development (Projects No. 2022GDASZH-2022010106, 2020GDASYL-20200102014, 2020GDASYL-20200102015, 2020GDASYL-20200102018, 2019GDASYL-0102002-1) , and Guangdong Foundation for Program of Science and Technology Research (Project No. 2023B1212060044) .

语种：英文

外文关键词：Heavy metal; Plant growth; Keystone taxa; Metabolic potentials

摘要：Heavy metal (HM) contamination of agricultural products is of global environmental concern as it directly threatened the food safety. Plant-associated microbiome, particularly endophytic microbiome, hold the potential for mitigating HM stress as well as promoting plant growth. The metabolic potentials of the endophytes, especially those under the HM stresses, have not been well addressed. Rice, a major staple food worldwide, is more vulnerable to HM contamination compared to other crops and therefore requires special attentions. Therefore, this study selected rice as the target plants. Geochemical analysis and amplicon sequencing were combined to characterize the rice root endophytic bacterial communities and identify keystone taxa in two HM-contaminated rice fields. Metagenomic analysis was employed to investigate the metabolic potentials of these keystone taxa. Burkholderiales and Rhizobiales were identified as predominant keystone taxa. The metagenome-assembled genome (MAG)s associated with these keystone populations suggested that they possessed diverse genetic potentials related to metal resistance and transformation (e.g., As resistance and cycling, V reduction, Cr efflux and reduction), and plant growth promotion (nitrogen fixation, phosphate solubilization, oxidative stress resistance, indole-3-acetic acid, and siderophore production). Moreover, bacteriophages encoding auxiliary metabolism genes (AMGs) associated with the HM resistance as well as nitrogen and phosphate acquisition were identified, suggesting that these phages may contribute to these crucial biogeochemical processes within rice roots. The current findings revealed the beneficial roles of rice endophytic keystone taxa and their associated bacteriophages within HM-contaminated rice root endophytic microbiome, which may provide valuable insights on future applications of employing root microbiome for safety management of agriculture productions.