文献类型：期刊文献

英文题名：Development and Characterization of a New TILLING Population for Forward and Reverse Genetics in Barley (Hordeum vulgare L.)

作者：Wang, Feifei Zhu, Liang Zhou, Zhenxiang Gu, Yangyang Guo, Baojian Lv, Chao Zhu, Juan Liu, Xiaohui Xu, Rugen

第一作者：Wang, Feifei

通信作者：Xu, RG[1];Liu, XH[2]

机构：[1]Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China;[2]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China

通信机构：corresponding author), Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China;corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2024

卷号：13

期号：17

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001312205100001)】；

基金：This project is supported by the National Natural Science Foundation of China (32101652); the Young Scientists Fund of the National Natural Science Foundation of China (32001463).

语种：英文

外文关键词：barley; mutagenesis; TILLING; mutant phenotype; HvGLR3.5

摘要：Mutagenesis is an important tool in crop improvement and free of the regulatory restrictions imposed on genetically modified organisms. Barley (Hordeum vulgare L.) is a diploid species with a genome smaller than those of other members of the Triticeae crops, making it an attractive model for genetic studies in Triticeae crops. In this study, we report an ethyl methane sulfonate (EMS)-mutagenized population in the Chinese barley landrace TX9425, which is tolerant to both abiotic and biotic stress. A TILLING (Targeting Induced Locus Lesion in Genomes) population consisting of 2000 M-2 lines was also constructed based on the CEL I enzyme with subsequent polyacrylamide electrophoresis, which decreased the cost and labor investment. The mutant phenotypes of the M-2 and M-3 generations were scored and revealed the presence of a wide spectrum of morphological diversity. The population was evaluated by screening for induced mutations in five genes of interest. A detailed analysis was performed for the HvGLR3.5 gene and three mutations were identified by screening in 2000 M-2 lines. Two of three mutations displayed tuft and yellow striped leaves compared to the wild type. Altogether, our study shows the efficiency of screening and the great potential of the new TILLING population for genetic studies in the barley crop model system.