文献类型：期刊文献

英文题名：A simple and label-free fluorescent DNA sensor for visual detection of aptamer-based berberine

作者：Zhang, Wenjuan Luo, Mingwan Chen, Juan Li, Zhengxing Wei, Xian Wu, Miqi Yang, Shengli He, Yuanju Wang, Xiaoping Xiao, Zhiyou

第一作者：张文娟

通信作者：Xiao, ZY[1]|[14440ef2cf80e422d3de7]肖志友;

机构：[1]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550025, Peoples R China

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Chem Engn, Guiyang 550025, Peoples R China.|贵州理工学院化学工程学院;贵州理工学院;

年份：2024

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20244117163605);Scopus(收录号：2-s2.0-85205683497);WOS:【SCI-EXPANDED(收录号:WOS:001314585500001)】；

基金：The study was supported by the National Natural Science Foundation of China (No. 21768002), Science and Technology Foundation of Guizhou Province (Qiankehe Jichu ZK[2021]077), and Scientific Research Foundation for Advanced Talents of Guizhou Institute of Technology (XJGC20190628).

语种：英文

摘要：The intrinsic fluorescence of berberine is very weak, which can be enhanced by its interaction with specific aptamers. A simple and sensitive DNA sensor for visual detection of berberine has here been established. When using this sensor, there was a good linear relationship between the change in fluorescence intensity of berberine and the concentration of berberine in the range of 16-2000 nM, with a detection limit of 5.1 nM. The change in fluorescence intensity was caused by the addition of aptamers. A detection limit of 170.1 nM was acquired by reading the RGB values of fluorescent images with a smartphone for the quantification of berberine. Common antibiotics did not interfere with the measurement of the berberine concentration. The molecular ion peaks of the complexes formed by the aptamer and berberine could be clearly observed by electrospray ionization mass spectrometry. The UV-vis absorption spectra, circular dichroism spectra, and fluorescence spectra indicated a strong interaction between berberine and the aptamer. The dissociation constant (Kd) between berberine and the aptamer was 1.91 mu M. This sensor was both simple and sensitive, requiring only a 21-base oligonucleotide. It realized a visual quantitative analysis with a smartphone. This method could also be used for similar fluorescence visualization determination of aptamer-based drug molecules. A simple and label-free fluorescent DNA sensor for visual detection of berberine through the interaction with an aptamer.