文献类型：期刊文献

英文题名：Nanopriming with selenium doped carbon dots improved rapeseed germination and seedling salt tolerance

作者：Khan, Mohammad Nauman Fu, Chengcheng Liu, Xiaohui Li, Yanhui Yan, Jiasen Yue, Lin Li, Jiaqi Khan, Zaid Nie, Lixiao Wu, Honghong

第一作者：Khan, Mohammad Nauman

通信作者：Wu, HH[1];Wu, HH[2];Nie, LX[3];Wu, HH[4];Wu, HH[5]

机构：[1]Huazhong Agr Univ, Ctr Crop Nanobiotechnol, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Hubei, Peoples R China;[2]Hubei Hongshan Lab, Wuhan 430070, Hubei, Peoples R China;[3]Hainan Univ, Sanya Inst Breeding & Multiplicat, Sch Breeding & Multiplicat, Sanya 572000, Hainan, Peoples R China;[4]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Guizhou, Peoples R China;[5]South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China;[6]Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Shenzhen 511464, Guangdong, Peoples R China;[7]Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr,Shenzhen Branch, Shenzhen 511464, Guangdong, Peoples R China

第一机构：Huazhong Agr Univ, Ctr Crop Nanobiotechnol, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Hubei, Peoples R China

通信机构：corresponding author), Huazhong Agr Univ, Ctr Crop Nanobiotechnol, Natl Key Lab Germplasm Innovat & Utilizat Hort Cro, MOA Key Lab Crop Ecophysiol & Farming Syst Middle, Wuhan 430070, Hubei, Peoples R China;corresponding author), Hubei Hongshan Lab, Wuhan 430070, Hubei, Peoples R China;corresponding author), Hainan Univ, Sanya Inst Breeding & Multiplicat, Sch Breeding & Multiplicat, Sanya 572000, Hainan, Peoples R China;corresponding author), Huazhong Agr Univ, Shenzhen Inst Nutr & Hlth, Shenzhen 511464, Guangdong, Peoples R China;corresponding author), Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Genome Anal Lab,Minist Agr,Shenzhen Branch, Shenzhen 511464, Guangdong, Peoples R China.

年份：2024

卷号：12

期号：5

起止页码：1333-1343

外文期刊名：CROP JOURNAL

收录：;Scopus(收录号：2-s2.0-85192162392);WOS:【SCI-EXPANDED(收录号:WOS:001358658000001)】；

基金：We thank Prof. Jimei Ma, Mr. Jianbo Cao and Ms. Limin He for their help in the preparation of CDs and TEM imaging at Public Laboratory of Electron Microscopy, Huazhong Agricultural University. This work was supported by the National Natural Science Foutndation of China (32071971, 32001463) , the National Key Research and Development Program of China (2022YFD2300205) , Fundamental Research Funds for the Central Universities (2662023ZKPY002) , the HZAU-AGIS Cooperation Fund (SZYJY2021008) , the Key Research and Development Projects of Henan province (231111113000) , and the Hubei Agricultural Science and Technology Innovation Center Program (2021-620-000-001-032) . Lixiao Nie would like to thank Hainan Major Science and Technology Projects (ZDKJ202001) . ICP-OES was completed by Shiyanjia Lab ( www.shiyanjia.com ) .

语种：英文

外文关键词：PAA@Se-CDs; Nanopriming; Salt stress; Antioxidant system; ATP production; K*/Na* balance

摘要：Soil salinity is a big environmental issue affecting crop production. Although seed nanopriming has been widely used to improve seed germination and seedling growth under salinity, our knowledge about the underlying mechanisms is still insufficient. Herein, we newly synthesized selenium-doped carbon dots nanoparticles coated with poly acrylic acid (poly acrylic acid coated selenium doped carbon dots, PAA@Se-CDs) and used it to prime seeds of rapeseeds. The TEM (transmission electron microscope) size and zeta potential of PAA@Se-CDs are 3.8 +/- 0.2 nm and- 30 mV, respectively. After 8 h priming, the PAA@Se-CDs nanoparticles were detected in the seed compartments (seed coat, cotyledon, and radicle), while no such signals were detected in the NNP (no nanoparticle control) group (SeO2 was used as the NNP). Nanopriming with PAA@Se-CDs nanoparticles increased rapeseeds germination (20%) and seedling fresh weight (161%) under saline conditions compared to NNP control. PAA@Se-CDs nanopriming significantly enhanced endo-b-mannanase activities (255% increase, 21.55 mu mol h- 1 g-1 vs. 6.06 mu mol h-1 g-1, at DAS 1 (DAS, days after sowing)), total soluble sugar (33.63 mg g-1 FW (fresh weight) vs. 20.23 mg g-1 FW) and protein contents (1.96 mu g g-1 FW vs. 1.0 mu g g-1 FW) to support the growth of germinating seedlings of rapeseeds under salt stress, in comparison with NNP control. The respiration rate and ATP content were increased by 76% and 607%, respectively. The oxidative damage of salinity due to the over- accumulation of reactive oxygen species (ROS) was alleviated by PAA@Se-CDs nanopriming by increasing the antioxidant enzyme activities (SOD (superoxide dismutase), POD (peroxidase), and CAT (catalase)). Another mechanism behind PAA@Se-CDs nanopriming improving rapeseeds salt tolerance at seedling stage was reducing sodium (Na*) accumulation and improving potassium (K*) retention, hence increasing the K*/Na* ratio under saline conditions. Overall, our results not only showed that seed nanopriming with PAA@Se-CDs could be a good approach to improve salt tolerance, but also add more knowledge to the mechanism behind nanopriming-improved plant salt tolerance at germination and early seedling growth stage. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).