文献类型：期刊文献

英文题名：Synthesis and Screening of Chemical Agents Targeting Viral Protein Genome-Linked Protein of Telosma Mosaic Virus

作者：Zhao, Guili Zhang, Wenjuan Fu, Xiaodong Xie, Xin Bai, Song Li, Xiangyang

第一作者：Zhao, Guili;赵贵丽

通信作者：Bai, S[1];Bai, S[2];Li, XY[2];Bai, S[3]|[1444086c12c707ddfe99e]柏松;

机构：[1]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China;[2]Guizhou Univ, Natl Key Lab Green Pesticide, Key Lab Green Pesticide & Agr Bioengn, Minist Educ, Guiyang 550025, Peoples R China;[3]Guizhou Univ, Coll Agr, Key Lab Agr Microbiol, Guiyang 550025, Peoples R China;[4]Guizhou Ind Polytech Coll, Guiyang 550008, Peoples R China

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China;corresponding author), Guizhou Univ, Natl Key Lab Green Pesticide, Key Lab Green Pesticide & Agr Bioengn, Minist Educ, Guiyang 550025, Peoples R China;corresponding author), Guizhou Ind Polytech Coll, Guiyang 550008, Peoples R China.|贵州理工学院化学工程学院;贵州理工学院;

年份：2023

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20234014825160);Scopus(收录号：2-s2.0-85171900189);WOS:【SCI-EXPANDED(收录号:WOS:001064320100001)】；

基金：This work was supported in part by the Science and Technology Planning Project of Guizhou Province (grant no. Qian Ke He Ji Chu ZK [2022] Zhong Dian 025), High School Science and Technology Talent Support Project of Guizhou Province, China (grant no. Qian Jiao He KY Zi [2021] 037), Science and Technology Support Project of Guizhou Province (grant no. Qia n Ke He Zhi Cheng [2020] 1Y135), Academic new seedling cultivation and exploration and innovation project of Guizhou Institute of Technology (grant no. GZLGXM-14), High-Level Talent Initial Funding of Guizhou Institute of Technology (grant no. XJGC20190632), Earth Papers Project of Guizhou Institute of Technology (grant no. KJZX20-005), the Opening Foundation of the Key Laboratory of Green Pesticide and Agricultural Bioengineering, the Ministry of Education, Guizhou University (grant no. Qian Jiao Ji [2022] 433), the National Key Research and Development Program of China (grant no. 2021YFD1700101) and National Natural Science Foundation of China (grant no. 31960546 and 32172461), Talents of Guizhou Science and Technology Cooperation Platform [grant no. (2021)5623], Guizhou Science and Technology Cooperation Foundation [grant no. ZK(2021)140], the Unique Feature Project of Guizhou Provincial Education Department [grant no. KY(2021)056], Program of Introducing Talents of Discipline to Universities of China (grant no. 111 program, D20023).

语种：英文

外文关键词：telosma mosaic virus(TeMV); viral protein genome-linkedprotein (VPg); benzenesulfonamide derivatives; interaction; antiviral activities

摘要：The viral protein genome-linked protein (VPg) of telosma mosaic virus (TeMV) plays an important role in viral reproduction. In this study, the expression conditions of TeMV VPg were explored. A series of novel benzenesulfonamide derivatives were synthesized. The binding sites of the target compounds and TeMV VPg were studied by molecular docking, and the interaction was verified by microscale thermophoresis. The study revealed that the optimal expression conditions for TeMV VPg were in Escherichia coli Rosetta with IPTG concentration of 0.8 mM and induction temperature of 25 degrees C. Compounds A4, A6, A9, A16, and A17 exhibited excellent binding affinity to TeMV VPg, with K-d values of 0.23, 0.034, 0.19, 0.086, and 0.22 mu M, respectively. LYS 121 is the key amino acid site. Compounds A9 inhibited the expression of TeMV VPg in Nicotiana benthamiana. The results suggested that TeMV VPg is a potential antiviral target to screen anti-TeMV compounds.