文献类型：期刊文献

英文题名：A Saprophytic Fungus Tubeufia rubra Produces Novel Rubracin D and E Reversing Multidrug Resistance in Cancer Cells

作者：Qian, Shengyan Zeng, Xuebo Qian, Yixin Lu, Yongzhong He, Zhangjiang Kang, Jichuan

第一作者：Qian, Shengyan

通信作者：He, ZJ[1];Kang, JC[1];Kang, JC[2]

机构：[1]Guizhou Univ, Engn & Res Ctr Southwest Biopharmaceut Resources, Natl Educ Minist China, Guiyang 550025, Peoples R China;[2]Guizhou Univ, Sch Life Sci, Guiyang 550025, Peoples R China;[3]Zunyi Med Univ, Guizhou Key Lab Characterist Microbial Res & Drug, Zunyi 563000, Peoples R China;[4]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Engn & Res Ctr Southwest Biopharmaceut Resources, Natl Educ Minist China, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Engn & Res Ctr Southwest Biopharmaceut Resources, Natl Educ Minist China, Guiyang 550025, Peoples R China;corresponding author), Guizhou Univ, Sch Life Sci, Guiyang 550025, Peoples R China.

年份：2023

卷号：9

期号：3

外文期刊名：JOURNAL OF FUNGI

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000958246600001)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC Grants Nos. 32170019 and 31670027), the Open Fund Program of Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of National Education Ministry of China, Guizhou University (No. GZUKEY201606) and the Science and Technology Foundation of Guizhou Province ([2020]1Y058).

语种：英文

外文关键词：Tubeufia rubra; secondary metabolites; Glyceroglycolipids; cancer multidrug resistance; reverse; P-glycoprotein

摘要：Two new (1, 2 viz Rubracin D and E) and sixteen known Glyceroglycolipids (3-18) in the saprophytic fungus Tubeufia rubra (PF02-2) from decaying wood in freshwater habitat were isolated and identified. Their chemical structures were elucidated via means of the extensive spectroscopic analyses of NMR, HR-ESI-MS and UV spectra, as well as comparison with literature data. The new compounds were assayed for the reversal activity of multidrug resistance (MDR) on MCF-7/ADM, K562/ADM and A549/ADM cell lines, and both compounds 1 and 2 reversed MDR in the three resistant cancer cell lines with concentration dependence. In the assay on K562/ADM, both new compounds had been proved to have remarkable MDR reversal effects, which were higher than those of the positive control viz Verapamil (Vrp). Meanwhile, in the assay on A549/ADM, compound 1 displayed significant MDR reversal effects, which were also higher than those of Vrp at certain concentrations. Furthermore, the Western blot assay proved that both new compounds reversed the MDR in the resistant cancer cell line viz MCF-7/ADM by inhibiting the overexpression of P-glycoprotein. This is the first report that the Glyceroglycolipids isolated firstly from the fungal genus Tubeufia reversed MDR in resistant cancer cells.