文献类型：期刊文献

英文题名：3D Printing of Gelatin-Based Hydrogels for Mimicking the Mechanical Properties of Ultrasoft Tissues

作者：Shi, Wenhai Chen, Yuewei Li, Yi Xie, Mingjun Li, Jiachun Zou, Zhongfei Liu, Xixia

第一作者：Shi, Wenhai

通信作者：Li, Y[1];Li, JC[1];Xie, MJ[2]

机构：[1]Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China;[2]Guizhou Inst Technol, Sch Mech Engn, Guiyang 550003, Peoples R China;[3]Zhejiang Univ, Affiliated Hosp 1, Sch Med, Dept Orthoped, Hangzhou 310003, Peoples R China

第一机构：Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China;corresponding author), Zhejiang Univ, Affiliated Hosp 1, Sch Med, Dept Orthoped, Hangzhou 310003, Peoples R China.

年份：2025

卷号：7

期号：12

起止页码：7729-7738

外文期刊名：ACS APPLIED POLYMER MATERIALS

收录：;EI(收录号：20252518634677);Scopus(收录号：2-s2.0-105008308751);WOS:【SCI-EXPANDED(收录号:WOS:001512666500001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (No. 52465035, No. 52305300), the Science and Technology Planning Project of Guizhou Province (No. ZK [2024] 510), the Guizhou Provincial Basic Research Program (Natural Science) (No. MS [2025] 615, No. MS [2025] 621, No. MS [2025]581), and the Guizhou Provincial Department of Science and Technology Project (No. [2022] 196, No. [2024] 020).

语种：英文

外文关键词：light-cured 3D printing; gelatin methacrylate (GelMA); ultrasoft scaffolds; mechanically matched; molecular network modulation; composite bioinks

摘要：In ultrasoft tissue regeneration, the mechanical properties of implanted scaffolds do not correspond with those of ultrasoft tissues and are prone to inflammation, fibrosis, and other comorbidities. Light-cured 3D-printed gelatin methacrylate (GelMA) scaffolds are widely used in soft tissue regeneration due to their excellent biocompatibility. However, during the printing of ultrasoft GelMA, defects such as delamination, damage, and fracture are prone to occur due to process factors such as interlayer peeling forces, which lead to molding difficulties. In this study, a GelMA/Collagenase II composite bioink was developed, in which GelMA with favorable mechanical properties was used to ensure the formation of the scaffolds; Collagenase II was used to shear the peptide bonds of the GelMA molecular chain to regulate the degree of sparseness of the molecular network, thereby softening the mechanical properties of the scaffolds. Combining finite element analysis and experimental verification, the compression modulus of GelMA was reduced from 18 to 1 kPa by regulating the softening time. Porous structures with tunable mechanical properties (1.4 kPa-0.1 kPa) were printed, and ultrasoft material modulus (1 kPa) molding of multiple structures (conduit, vascular, solid) was achieved while demonstrating excellent cell proliferation. This work provides a bioink for the 3D printing of ultrasoft bioscaffolds, which has great potential in the field of ultrasoft tissue repair.