文献类型：期刊文献

英文题名：The Denitrification Characteristics of Pseudomonas stutzeri SC221-M and Its Application to Water Quality Control in Grass Carp Aquaculture

作者：Deng, Bin Fu, Luoqin Zhang, Xiaoping Zheng, Jiajia Peng, Lisha Sun, Jiandong Zhu, Haiyan Wang, Yibing Li, Weifen Wu, Xuexiang Wu, Di

第一作者：邓斌;Deng, Bin

通信作者：Zhang, XP[1]

机构：[1]Zhejiang Univ, Coll Anim Sci, Inst Anim Nutr & Feed Sci, Key Lab Mol Feed Sci, Hangzhou 310003, Zhejiang, Peoples R China;[2]Guizhou Inst Technol, Sch Pharmaceut Engn, Guiyang, Peoples R China;[3]Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310003, Zhejiang, Peoples R China;[4]Guizhou Univ, Coll Anim Sci, Guiyang 550003, Peoples R China;[5]Ctr Dis Control & Prevent, Deyang, Peoples R China

第一机构：Zhejiang Univ, Coll Anim Sci, Inst Anim Nutr & Feed Sci, Key Lab Mol Feed Sci, Hangzhou 310003, Zhejiang, Peoples R China

通信机构：corresponding author), Zhejiang Univ, Coll Anim Sci, Inst Anim Nutr & Feed Sci, Key Lab Mol Feed Sci, Hangzhou 310003, Zhejiang, Peoples R China.

年份：2014

卷号：9

期号：12

外文期刊名：PLOS ONE

收录：;Scopus(收录号：2-s2.0-84918514159);WOS:【SCI-EXPANDED(收录号:WOS:000347515300092)】；

基金：This work was supported by National Basic Research Program, P. R. China (973 Program) 2009CB118705, Special Fund for Agro-scientific Research in the Public Interest, 201203083, and Foundation of Agricultural Science and Technology Support Project of Guizhou Province [2010] 3053. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：To reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium Pseudomonas stutzeri, SC221-M, was obtained. The effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirS and nosZ in SC221-M were studied. The following conditions were determined to be optimal for growth and denitrification in SC221-M: NaNO2 as the nitrogen source, sodium citrate as the carbon source, a carbon to nitrogen ratio range of 4-8, and a temperature range of 20-35 degrees C. Subsequently, SC221-M and the Bacillus cereus BSC24 strain were selected to generate microbial preparations. The results showed that addition of the microbial preparations decreased various hydrochemical parameters, including total dissolved solids, ammonium, nitrite, total nitrogen and the chemical oxygen demand. Nitrogen removal rates were highest on day 9; the removal rates of BSC24, SC221-M, a mixed preparation and a 3 x mixed preparation were 24.5%, 26.6%, 53.9% and 53.4%, respectively. The mixed preparation (SC221-M+BSC24) was more effective at removing nitrogen than either the SC221-M or BSC24 preparation. Roche 454 pyrosequencing and subsequent analysis indicated that the control and other groups formed separate clusters, and the microbial community structure in the water changed significantly after the addition of microbial preparations. These results indicate that the addition of microbial preparations can improve both the water quality and microbial community structure in an experimental aquaculture system. P. stutzeri strain SC221-M and its related microbial preparations are potential candidates for the regulation of water quality in commercial aquaculture systems.