文献类型：期刊文献

英文题名：Identification and Characterization of Peptaibols as the Causing Agents of Pseudodiplo?spora longispora Infecting the Edible Mushroom Morchella

作者：Dong, Wang Chen, Baosong Zhang, Rui Dai, Huanqin Han, Junjie Lu, Yongzhong Zhao, Qi Liu, Xingzhong Liu, Hongwei Sun, Jingzu

第一作者：Dong, Wang

机构：[1] State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Chaoyang District, Beijing, 100101, China; [2] School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, China; [3] School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang, 550003, China; [4] Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Science, Kunming, 650201, China; [5] Department of Microbiology, College of Life Science, Nankai University, Jinnan District, Tianjin, 300350, China; [6] Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China

第一机构：State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 3 Park 1, Beichen West Road, Beijing, 100101, China

年份：2023

外文期刊名：Journal of Agricultural and Food Chemistry

收录：EI(收录号：20234815112458);Scopus(收录号：2-s2.0-85177817490)

语种：英文

外文关键词：Aspergillus

摘要：Pseudodiplo?spora longispora (previously known as Diplo?spora longispora) is a pathogenic fungus of Morchella mushrooms. The molecular mechanism underlying the infection of P. longispora in fruiting bodies remains unknown. In this study, three known peptaibols, alamethicin F-50, polysporin B, and septocylindrin B (1-3), and a new analogue, longisporin A (4), were detected and identified in the culture of P. longispora and the fruiting bodies of M. sextelata infected by P. longispora. The primary amino sequence of longisporin A is defined as Ac-Aib1-Pro2-Aib3-Ala4-Aib5-Aib6-Gln7-Aib8-Val9-Aib10-Glu11-Leu12-Aib13-Pro14-Val15-Aib16-Aib17-Gln18-Gln19-Phaol20. The peptaibols 1-4 greatly suppressed the mycelial growth of M. sextelata. In addition, treatment with alamethicin F-50 produced damage on the cell wall and membrane of M. sextelata. Compounds 1-4 also exhibited inhibitory activities against human pathogens including Aspergillus fumigatus, Candida albicans, methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus, and plant pathogen Verticillium dahlia. Herein, peptaibols are confirmed as virulence factors involved in the invasion of P. longispora on Morchella, providing insights into the interaction between pathogenic P. longispora and mushrooms. ? 2023 American Chemical Society.