文献类型：期刊文献

英文题名：Nitric oxide enhances ethanol stress tolerance in Wickerhamomyces anomalus through modulating cell membrane and cell wall homeostasis

作者：Liu, Xiaozhu Wang, Yujie Lu, Jinzhou Zhang, Xuewen Li, Yinfeng

通信作者：Li, YF[1]

机构：[1]Guizhou Inst Technol, Guiyang 550000, Peoples R China;[2]Hunan Agr Univ, Changsha 410128, Peoples R China;[3]Zhejiang Xitang Old Wine Co Ltd, Jiaxing 314001, Peoples R China

第一机构：贵州理工学院

通信机构：corresponding author), Guizhou Inst Technol, Guiyang 550000, Peoples R China.|贵州理工学院;

年份：2026

卷号：26

期号：1

外文期刊名：BMC MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-105040841219);WOS:【SCI-EXPANDED(收录号:WOS:001785884700001)】；

基金：This work was supported by Guizhou Provincial Science and Technology Foundation (Qiankehejichu MS [2026]244; [2025]192).

语种：英文

外文关键词：Nitric oxide; Ethanol stress; Wickerhamomyces anomalus; Cell wall; Cell membrane

摘要：Background Wickerhamomyces anomalus has garnered significant interest for its remarkable ability to shape wine flavor profiles. However, during fermentation, yeast cells are inevitably exposed to ethanol stress. Nitric oxide (NO) is a key signaling molecule that plays diverse physiological roles in living organisms. However, its impact on cell wall and membrane homeostasis under ethanol stress remains unclear. Results In this study, we investigated the regulatory effects of NO on the cellular integrity of W. anomalus under ethanol stress by supplementing with the NO donor (SNP) or the NO scavenger (Carboxy-PTIO) using physiological analyses focused on the cell wall and membrane. Our results demonstrated that elevated NO levels alleviated ethanol-induced morphological and ultrastructural alterations, primarily by maintaining cellular homeostasis, ultimately promoting cell survival and vitality. Specifically, NO mitigated ethanol stress-compromised cell wall integrity by activating the cell wall integrity (CWI) pathway and increasing intracellular levels of beta-glucan and chitin. Furthermore, NO alleviated ethanol-induced disruption of membrane homeostasis by remodeling membrane composition, enhancing integrity, permeability, and fluidity, while simultaneously increasing ATPase activity, elevating intracellular K+ levels, and regulating fatty acid synthesis. Conclusions These findings provide crucial insights into the mechanistic basis of NO-regulated stress responses in W. anomalus under ethanol stress and offer a foundation for developing novel strategies to improve its industrial utilization under fermentative stress conditions.