文献类型：期刊文献

英文题名：Transcriptome and metabolome analyses reveal molecular insights into waterlogging tolerance in Barley

作者：Wang, Feifei Zhou, Zhenxiang Liu, Xiaohui Zhu, Liang Guo, Baojian Lv, Chao Zhu, Juan Chen, Zhong-Hua Xu, Rugen

第一作者：Wang, Feifei

通信作者：Xu, RG[1]

机构：[1]Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China;[2]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China;[3]Western Sydney Univ, Hawkesbury Inst Environm, Sch Sci, Penrith, NSW 2751, Australia

第一机构：Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China

通信机构：corresponding author), Yangzhou Univ, Inst Agr Sci, Jiangsu Coinnovat Ctr Modern Prod Technol Grain Cr, Key Lab Plant Funct Genom,Minist Educ,Jiangsu Key, Yangzhou 225009, Peoples R China.

年份：2024

卷号：24

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85192562013);WOS:【SCI-EXPANDED(收录号:WOS:001217621400008)】；

基金：This project is supported by National Natural Science Foundation of China (32101652); Young Scientists Fund of the National Natural Science Foundation of China (32001463); Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD); Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX22_3518).

语种：英文

外文关键词：Hordeum vulgare L.; Hypoxia; Waterlogging stress; Metabolites; Multi-omics; Phenylpropanoid biosynthesis; Ethanol fermentation

摘要：Waterlogging stress is one of the major abiotic stresses affecting the productivity and quality of many crops worldwide. However, the mechanisms of waterlogging tolerance are still elusive in barley. In this study, we identify key differentially expressed genes (DEGs) and differential metabolites (DM) that mediate distinct waterlogging tolerance strategies in leaf and root of two barley varieties with contrasting waterlogging tolerance under different waterlogging treatments. Transcriptome profiling revealed that the response of roots was more distinct than that of leaves in both varieties, in which the number of downregulated genes in roots was 7.41-fold higher than that in leaves of waterlogging sensitive variety after 72 h of waterlogging stress. We also found the number of waterlogging stress-induced upregulated DEGs in the waterlogging tolerant variety was higher than that of the waterlogging sensitive variety in both leaves and roots in 1 h and 72 h treatment. This suggested the waterlogging tolerant variety may respond more quickly to waterlogging stress. Meanwhile, phenylpropanoid biosynthesis pathway was identified to play critical roles in waterlogging tolerant variety by improving cell wall biogenesis and peroxidase activity through DEGs such as Peroxidase (PERs) and Cinnamoyl-CoA reductases (CCRs) to improve resistance to waterlogging. Based on metabolomic and transcriptomic analysis, we found the waterlogging tolerant variety can better alleviate the energy deficiency via higher sugar content, reduced lactate accumulation, and improved ethanol fermentation activity compared to the waterlogging sensitive variety. In summary, our results provide waterlogging tolerance strategies in barley to guide the development of elite genetic resources towards waterlogging-tolerant crop varieties.