文献类型：期刊文献

英文题名：Biological Reinforced Concrete for Cartilage Repair With 3D Printing

作者：Chen, Yuewei Fu, Tao Zou, Zhongfei Liu, Yanming Zhu, Jianguo Teng, Binhong Yao, Ke Li, Haibin Li, Jiachun Xie, Zhijian He, Yong

第一作者：Chen, Yuewei

通信作者：Li, JC[1];He, Y[2];He, Y[3];Xie, ZJ[4]

机构：[1]Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China;[2]Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;[3]Zhejiang Univ, Sch Mech Engn, Liangzhu Lab, Hangzhou 310027, Peoples R China;[4]Zhejiang Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Affiliated Hosp 2,Sch Med, Hangzhou 310000, Zhejiang, Peoples R China;[5]Key Lab Oral Biomed Res Zhejiang Prov, Hangzhou 310000, Zhejiang, Peoples R China;[6]Zhejiang Univ, Engn Res Ctr Oral Biomat & Devices Zhejiang Prov, Zhejiang Prov Clin Res Ctr Oral Dis, Sch Med,Canc Ctr,Sch Stomatol,Key Lab Oral Biomed, Hangzhou 310006, Peoples R China;[7]Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Orthodont, Hangzhou 310000, Zhejiang, Peoples R China;[8]Guizhou Univ, Guizhou Prov Peoples Hosp, Affiliated Hosp, Dept Urol, Guiyang 550002, Guizhou, Peoples R China;[9]Guizhou Inst Technol, Sch Mech Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China;corresponding author), Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, Sch Mech Engn, Liangzhu Lab, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, Engn Res Ctr Oral Biomat & Devices Zhejiang Prov, Zhejiang Prov Clin Res Ctr Oral Dis, Sch Med,Canc Ctr,Sch Stomatol,Key Lab Oral Biomed, Hangzhou 310006, Peoples R China.

年份：2025

外文期刊名：ADVANCED SCIENCE

收录：;EI(收录号：20251017991639);Scopus(收录号：2-s2.0-85219532504);WOS:【SCI-EXPANDED(收录号:WOS:001430461500001)】；

基金：Y.C., T.F., and Z.Z. are co-first authors. Y.C., T.F., and Z.Z. contributed equally to this work. This work was sponsored by the National Natural Science Foundation of China (Nos. 52465035, 52325504, 52235007, T2121004), the Science and Technology Planning Project of Guizhou Province (Nos. ZK[2024] 510, ZK[2025] 615, LH[2024]ZD 020, [2022]YB 196), Open Foundation of the State Key Laboratory of Fluid Power and Mechatronic Systems (No. GZKF-202404), Application Verification in Universities (No. BX2024B033), Key R&D Program of Zhejiang (No. 2024SSYS0027), Medical Healthy Scientific Technology Project of Zhejiang Province (No. 2022511252), and Zhejiang Province Natural Science Foundation of China (No. LQ23H090012).

语种：英文

外文关键词：3D-printed ultrafine fiber networks; biohydrogels; biological reinforced concrete; cartilage; extracellular matrix

摘要：The development of biomimetic cartilage constructs (BCCs) with natural extracellular matrix (ECM) microenvironments and topological cues to accelerate the reconstruction of natural articular cartilage (NAC) after injury is challenging due to its complex structure, low cellular content, and less vascularity. Inspired by concrete rebar structure, a biomimetic cartilage named "biological reinforced concrete" is fabricated, with collagen fiber orientation transitioning from parallel to perpendicular, replicating the ECM microenvironments and complex construct of NAC. 3D-printed ultrafine fiber networks (UFNs) served as a degradable "biorebars", while a hybrid biohydrogel acted as "biocement". The stem cells are utilized as "bioactive aggregates". The biocement is developed by combining and screening various biohydrogels to mimic an ECM microenvironment conducive to the formation of NAC. By adjusting the fiber scale and spacing of the UFNs, the mechanical properties of the biomimetic cartilages are controlled to resemble those of NAC. Additionally, the UFNs guided the directed growth of cells and the orderly secretion of ECM. Subsequently, the developed BCCs are implanted into an osteochondral defect, and after 4 months, they successfully reconstructed the complex structure of cartilage with mechanical properties closely resembling those of NAC. The biological reinforced concrete offers a customizable and universal strategy for tissue regeneration.