文献类型：期刊文献

英文题名：Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway

作者：Xu, Duoling Wang, Chao Wu, Jie Fu, Yuanxiang Li, Shujun Hou, Wentao Lin, Ling Li, Pei Yu, Dongsheng Zhao, Wei

第一作者：Xu, Duoling

通信作者：Yu, DS[1];Zhao, W[1];Yu, DS[2];Zhao, W[2]

机构：[1]Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Guangdong, Peoples R China;[3]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China;[4]Guizhou Inst Technol, Guizhou Prov Key Lab Energy Chem, Guiyang 550003, Peoples R China;[5]Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China

第一机构：Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China

通信机构：corresponding author), Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China;corresponding author), Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Guangdong, Peoples R China.

年份：2022

卷号：7

期号：16

起止页码：13546-13556

外文期刊名：ACS OMEGA

收录：;Scopus(收录号：2-s2.0-85128827841);WOS:【SCI-EXPANDED(收录号:WOS:000812740100001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (Nos. 81974146, 81873711), the Science and Technology Planning Project of Guangdong Province (2016A020215094), and the Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation ("Climbing Program" Special Funds, pdjh 2022b0012).

语种：英文

摘要：Graphene oxide quantum dots (GOQDs) are considered to be a new method for regulating the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are few reports on such regulation with different concentrations of GOQDs, and the molecular mechanism has not been fully elucidated. The purposes of this study were, first, to explore the effects of GOQDs on the proliferation and differentiation of BMSCs in vitro and in vivo, and, second, to provide a theoretical basis for the repair of bone defects. Live/Dead staining, EdU staining, immuno-fluorescence staining, alkaline phosphatase (ALP), western blotting, and qT-PCR were used for detecting the proliferation and differentiation of BMSCs after coculture with GOQDs of different concentrations. Hematoxylin and eosin (HE) staining and Van Gieson (VG) staining were used to detect new bone regeneration in vivo. The results showed that low-concentration GOQDs (0.1 and 1 mu g/mL) promoted the proliferation and differentiation of BMSCs. Compared with the 1 mu g/mL GOQD group, the 0.1 mu g/mL GOQD group had better ability to promote the proliferation and differentiation of BMSCs. HE and VG staining results showed the greatest proportion of new bone area on sandblasted, large-grit, and acid-etched (SLA)/GOQD scaffolds. Furthermore, the ratio of active beta-catenin and the phosphorylation level of GSK-3 beta (p-GSK-3 beta) increased after BMSCs treatment with 0.1 mu g/mL GOQDs. Low concentrations of GOQDs improved the osteogenic differentiation ability of BMSCs by activating the Wnt/beta-catenin signaling pathway.