文献类型：期刊文献

英文题名：Functional restoration of uterine architecture and fertility via a 3D-printed biomimetic scaffold: The role of macrophage-driven immunomodulation

作者：Wei, Qianqian Zhang, Jing Weng, Jiahao Lan, Jiawen He, Jing Chen, Yuewei Zou, Zhongfei He, Yong Wang, Yanpeng Shu, Jing

第一作者：Wei, Qianqian

通信作者：Wang, YP[1];Shu, J[1];He, Y[2];He, Y[3]

机构：[1]Zhejiang Univ, Affiliated Hosp 1, Ctr Reprod Med, Sch Med, Hangzhou, Zhejiang, Peoples R China;[2]Zhejiang Univ, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;[3]Zhejiang Univ, Sch Mech Engn, Liangzhu Lab, Hangzhou 310027, Peoples R China;[4]Zhejiang Prov Peoples Hosp, Affiliated Peoples Hosp, Hangzhou Med Coll, Ctr Reprod Med,Dept Gynecol, Hangzhou, Zhejiang, Peoples R China;[5]Hangzhou Med Coll, Sch Basic Med Sci & Forens Med, Hangzhou, Zhejiang, Peoples R China;[6]Zhejiang Univ, Affiliated Hosp 2, Hangzhou 310027, Peoples R China;[7]Zhejiang Univ, Coll Mech Engn, Zhejiang Key Lab Addit Mfg Technol & Equipment, Hangzhou 310027, Peoples R China;[8]Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China;[9]Guizhou Inst Technol, Sch Mech Engn, Guiyang 550003, Peoples R China

第一机构：Zhejiang Univ, Affiliated Hosp 1, Ctr Reprod Med, Sch Med, Hangzhou, Zhejiang, Peoples R China

通信机构：corresponding author), Zhejiang Univ, Affiliated Hosp 1, Ctr Reprod Med, Sch Med, Hangzhou, Zhejiang, Peoples R China;corresponding author), Zhejiang Univ, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, Sch Mech Engn, Liangzhu Lab, Hangzhou 310027, Peoples R China.

年份：2026

卷号：37

外文期刊名：MATERIALS TODAY BIO

收录：;EI(收录号：20261020200257);Scopus(收录号：2-s2.0-105031622342);WOS:【SCI-EXPANDED(收录号:WOS:001709408300002)】；

基金：This study was supported by National Key Research and Development Program of China (2024YFB4610100) ; National Natural Science Foundation of China (82301829, 52235007, T2121004, 52325504,52 465035,5256050459) ; Key R & D Program of Zhejiang (2024SSYS0027) ; Zhejiang Provincial Medical and Health Science and Technology Program (2023KY526) ; The Science and Technology Planning Project of Guizhou Province (No. ZK [2024] 510, No. ZK [2025] 615)

语种：英文

外文关键词：PLCL; Tissue engineering; Regenerative medicine; Uterine defect; Macrophage polarization

摘要：The structural complexity of the uterus makes identifying biomaterials and scaffold designs for supporting functional tissue regeneration challenging. Poly(L-lactide-co-caprolactone) (PLCL) has gained attention due to its biocompatibility and tunable degradation profile. In this study, a three-layered PLCL scaffold was fabricated via melt electrowriting (MEW) 3D printing to mimic the mechanical properties of the native uterine matrix. Using a mouse model of severe uterine injury, we demonstrated that the PLCL scaffold markedly promoted structural repair and functional recovery, as evidenced by improved pregnancy outcomes. Mechanistically, the scaffold enhanced macrophage recruitment and skewed their polarization toward an M2 phenotype, accompanied by upregulation of the hallmark genes SPP1, LGALS3, and TREM2. Furthermore, the scaffold stimulated parenchymal cell proliferation and migration and activated the JAK-STAT3 and YAP-Hippo signaling pathways, thereby establishing a pro-regenerative niche. Collectively, these findings highlight a scaffold-driven immunomodulatory mechanism that promotes uterine regeneration through macrophage polarization and activation of key signaling pathways.