文献类型：期刊文献

中文题名：基于荧光免疫分析的血清标志物抗原快速检测实验研究

英文题名：Rapid Detection of Serum Marker Antigen Based on Fluorescence Immunoassay

作者：马小彦 张泽望 汤霞

第一作者：马小彦

机构：[1]贵州理工学院食品药品制造工程学院;[2]贵州大学生命科学学院

第一机构：贵州理工学院食品药品制造工程学院

年份：2018

期号：5

起止页码：66-69

中文期刊名：科技通报

外文期刊名：Bulletin of Science and Technology

收录：CSTPCD

基金：贵州理工学院科学基金(XJ2K20130805)

语种：中文

中文关键词：荧光免疫分析;血清标志物;抗原;检测;实验

外文关键词：fluorescence immunoassay;serum markers;antigens;detection;experiments

摘要：针对传统血清标志物抗原快速检测实验,一直处在检测实验过程复杂,结果不明确的问题,提出并设计了基于荧光免疫分析方法进行血清标志物抗原快速检测实验,为乙型肝炎病毒的预防提供依据。选择某大型医院门诊与住院的乙型肝炎患者血清633份,另取50例健康血清作为对照,通过荧光免疫分析方法对血清标志物抗原进行检测。结果发现,血清标志物表面模式共有12种,男性是女性的2倍左右,大三阳在青少年中感染比率很高,城镇和农村受试者HBsAg、抗-Hbc阳性相差较大,父母是否携带HBV受试者血清不同模式阳性检出率相差很大,大三阳和小三阳抗原阳性率有统计学差异。采用荧光免疫分析得到的LLD、BLD值更低,FS值更高,ARM范围更广,与真实检测值之间的一致性程度更强,说明荧光免疫分析方法敏感度、可靠性范围以及检测准确性均很高。
According to the traditional serum marker antigen rapid detection experiment,has been in the test process is complex,the problem is not clear,proposed and designed fluorescence immunoassay method for rapid detection of serum marker antigen based on the experiment,for the prevention of hepatitis B virus provides the basis. A total of 633 hepatitis B patients in a large hospital were selected,and 50 healthy sera were taken as controls. The serum markers were detected by fluorescence immunoassay. The results showed that serum markers of surface model has 12,male or female 2 times,HBeAg infection rate is high in adolescents,urban and rural subjects of HBs Ag,anti-Hbc positive difference,whether parents carry the HBV subjects different patterns of serum positive rate difference,statistically significant the positive rate of HBe Ag and small three antigen. By immunofluorescence analysis of LLD,lower BLD value,higher FS,ARM wider and stronger and between the real detection value of the degree of consistency,the fluorescent immune analysis method of sensitivity,reliability and accuracy of the detection range is very high.