文献类型：期刊文献

英文题名：Synthesis of AIE polyethylene glycol-block-polypeptide bioconjugates and cell uptake assessments of their self-assembled nanoparticles

作者：Yu, Mingguang Zhong, Songjing Quan, Yusi Liu, Houhe Zhong, Junyang Jiang, Xinlin Wang, Yisi Ye, Guodong Ma, Chunping Huang, Yugang

第一作者：Yu, Mingguang

通信作者：Huang, YG[1];Huang, YG[2]

机构：[1]Foshan Univ, Sch Mat Sci & Energy Engn, Foshan 528000, Peoples R China;[2]Guangzhou Med Univ, Sch Pharmaceut Sci, Key Lab Mol Target & Clin Pharmacol Guangdong Pro, Guangzhou 511436, Guangdong, Peoples R China;[3]Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou 511436, Guangdong, Peoples R China;[4]Guizhou Inst Technol, Sch Mat & Met Engn, Guiyang 550003, Guizhou, Peoples R China

第一机构：Foshan Univ, Sch Mat Sci & Energy Engn, Foshan 528000, Peoples R China

通信机构：corresponding author), Guangzhou Med Univ, Sch Pharmaceut Sci, Key Lab Mol Target & Clin Pharmacol Guangdong Pro, Guangzhou 511436, Guangdong, Peoples R China;corresponding author), Guangzhou Med Univ, Affiliated Hosp 5, Guangzhou 511436, Guangdong, Peoples R China.

年份：2019

卷号：170

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20192507067733);Scopus(收录号：2-s2.0-85067278201);WOS:【SCI-EXPANDED(收录号:WOS:000478711200089)】；

基金：This work is financially supported by the National Natural Science Foundation of China (51403043), the Municipal College Research Program of Guangzhou Education Bureau (1201610202), the Open Funding of Guangzhou Medical University for College Students (S201910570009) and the High-Level Talent Start-Up Research Project of Foshan University (gg040945).

语种：英文

外文关键词：Aggregation induced emission; Synthetic polypeptide; Aggregation caused quenching; Monomeric emission; Cell uptake

摘要：A synthetic polypeptide-based bioconjugate bearing tetraphenylethylene (TPE) at the polypeptide side chains, named mPEG-PPLGgTPE, was synthesized by ring-opening polymerization (ROP) of alpha-amino acid-N-carboxyanhydride (NCA) along with post-polymerization modification method. Interestingly, the bioconjugate enabled both the aggregation induced emission (AIE) effect at 469 nm and the aggregation caused quenching (ACQ) effect around 365 nm depending on water fraction in the tetrahydrofuran/water (THF/H2O) mixed solution, and this feature was very different from the commonly used TPE-based AIE polymers. The strong emission at the 365 nm was a rarely seen monomeric emission for the TPE units. Further studies on the fluorescence spectra indicated that the monomeric emission was not related with the secondary structure of polypeptides, but caused by the TPE unit itself. The inPEG-PPLGgTPE could self-assemble to AIE nanoparticles (NPs) in water in a mean size of 253 nm with polyethylene glycol (PEG) as the shells in surface. In addition, HeLa cell uptake level of the NPs was unsatisfactory according to the cell imaging results, which was independent of the polypeptides conformation, the PEG length and the size of NPs.