文献类型：期刊文献

英文题名：Insights from the Endophytic Fungi in Amphisphaeria (Sordariomycetes): A. orixae sp. nov. from Orixa japonica and Its Secondary Metabolites

作者：Wang, Xiaojie Wanasinghe, Dhanushka N. Zhang, Jingyi Ma, Jian Zhou, Peifeng Zhang, Lijuan Lu, Yongzhong Zhang, Zhen

第一作者：Wang, Xiaojie

通信作者：Zhang, Z[1];Lu, YZ[2]

机构：[1]Guizhou Univ, Sch Liquor & Food Engn, Guiyang 550025, Peoples R China;[2]Guizhou Acad Sci, Guizhou Acad Testing & Anal, Guiyang 550014, Peoples R China;[3]Chinese Acad Sci, Kunming Inst Bot, Ctr Mt Futures, Kunming 654400, Peoples R China;[4]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550003, Peoples R China

第一机构：Guizhou Univ, Sch Liquor & Food Engn, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Acad Sci, Guizhou Acad Testing & Anal, Guiyang 550014, Peoples R China;corresponding author), Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550003, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2023

卷号：11

期号：5

外文期刊名：MICROORGANISMS

收录：;Scopus(收录号：2-s2.0-85160802592);WOS:【SCI-EXPANDED(收录号:WOS:000996681400001)】；

基金：This work was funded by the Youth Science and Technology Talent Development Project from the Guizhou Provincial Department of Education (QJHKYZ [2022]345), the Guizhou Province high-level talent innovation and entrepreneurship merit funding project (No. 202104), and the academic emerging project of Guizhou Institute of Technology (GZLGXM-15).

语种：英文

外文关键词：Amphisphaeriales; fungal taxonomy; hyphomycetes; isocoumarin; phylogeny

摘要：Endophytic fungi are a remarkably diverse group of microorganisms that have imperceptible associations with their hosts for at least a part of their life cycle. The enormous biological diversity and the capability of producing bioactive secondary metabolites such as alkaloids, terpenoids, and polyketides have attracted the attention of different scientific communities, resulting in numerous investigations on these fungal endophytes. During our surveys of plant-root-based fungi in the mountain areas of Qingzhen, Guizhou Province, several isolates of endophytic fungi were identified. In this study, a novel endophytic fungus was discovered in the roots of a medicinal plant (Orixa japonica) in Southern China and introduced as a new species (Amphisphaeria orixae) based on morphological evidence and molecular phylogenetic analysis (combined ITS and LSU sequence data). To the best of our knowledge, A. orixae is the first reported endophyte as well as the first hyphomycetous asexual morph in Amphisphaeria. A new isocoumarin, (R)-4,6,8-trihydroxy-5-methylisochroman-1-one (1), and 12 known compounds (2-13) were isolated from the rice fermentation products of this fungus. Using 1D- and 2D-NMR, mass spectrometry, and ECD studies, their structures were identified. The antitumor activity of these compounds was tested. Unfortunately, none of the compounds tested showed significant antitumor activity.