文献类型：期刊文献

中文题名：不同产地天山雪莲中绿原酸和芦丁含量测定及其煎煮提取工艺优化

英文题名：Content Determination of Chlorogenic Acid and Rutoside in Saussurea involucrate from Different Producing Areas and Optimization of Decoction and Extraction Technology

作者：王强 刘运权 王涛 刘学 朱晶晶 张亚洲

第一作者：王强

机构：[1]贵阳中医学院附属第二医院神经内科;[2]贵州理工学院制药工程学院

第一机构：贵阳中医学院附属第二医院神经内科,贵阳550001

年份：2016

卷号：27

期号：25

起止页码：3539-3541

中文期刊名：中国药房

外文期刊名：China Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：贵州省科学技术基金项目(No.黔科合J字[2014]2042号)

语种：中文

中文关键词：天山雪莲;不同产地;含量测定;芦丁;绿原酸;正交试验;煎煮提取工艺

外文关键词：Saussurea involucrate; Different producing areas; Content determination; Rutoside; Chlorogenic acid; Orthogonal test; Decoction and extraction technology

摘要：目的：测定不同产地天山雪莲中绿原酸和芦丁的含量,优化天山雪莲的煎煮提取工艺。方法：采用高效液相色谱法测定10批不同产地天山雪莲中绿原酸和芦丁的含量;以绿原酸和芦丁提取转移率的综合得分为指标,采用L_9（3^4）正交试验优化加水量、煎煮次数、煎煮时间的水平,并进行验证试验。结果：10批雪莲中绿原酸含量为0.380%～0.546%,芦丁含量为0.334%～0.617%;最优煎煮工艺条件为取天山雪莲药材100 g,浸泡20 min,煎煮3次,每次分别加12、10、10倍药材的水,分别煎煮45、30、30 min。验证试验中绿原酸和芦丁提取转移率分别为96.2%、89.3%（RSD分别为2.66%、3.31%,n=3）。结论：不同产地的天山雪莲有效成分含量存在一定的差异;优化后的煎煮提取工艺稳定、可行。
OBJECTIVE： To detect the contents of chlorogenic acid and rutoside in Saussurea involucrate, and to optimize the decoction and extraction technology of S. involucrate from different producing areas. METHODS ： The contents of chlorogenic acid and rutoside in 10 batches of S. involucrate from different producing areas were determined by HPLC. L9（3^4） orthogonal experiment was used to optimize the water amount, decoction times and decoction time using comprehensive score of extraction transport rate of chlorogenic acid and rutoside as index. The verification test was also conducted. RESULTS： The contents of chlorogenic acid and rutoside in 10 batches of S. involucrate were 0.380%-0.546% and 0.334%-0.617% ; the optimal decoction technology was as follows as the amout of crude material of S. involucrate 100 g, soaking for 20 min, decocting for 3 times, 12, 10 and 10 fold of water, decocting 45, 30 and 30 min, respectively. The extraction transfer rates of chlorogenic acid and rutoside were 96.2% （RSD= 2.66%, n=3） and 89.3% （RSD=3.31%, n=3） in verification test. CONCLUSIONS： For S. involucrate from different producing areas, the contents of effective components are different; optimized decoction and extraction technology is stable and feasible.