文献类型：期刊文献

中文题名：一株产酯异常威克汉姆酵母逆境胁迫耐受性与全基因组测序分析

英文题名：Stress tolerance and whole-genome sequencing of an ester-producing Wickerhamomyces anomalus strain

作者：王育捷 王德维 龚韬 刘双燕 李银凤 赵燕 刘晓柱 张学文

第一作者：王育捷

机构：[1]湖南农业大学生物科学技术学院,湖南长沙410128;[2]贵州理工学院食品药品制造工程学院,贵州贵阳550003

第一机构：湖南农业大学生物科学技术学院,湖南长沙410128

年份：2025

卷号：44

期号：5

起止页码：32-39

中文期刊名：中国酿造

外文期刊名：China Brewing

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金项目(32160557);贵州省自然科学基金项目(黔科合基础MS[2025]192,黔科合基础-ZK[2023]一般137)。

语种：中文

中文关键词：异常威克汉姆酵母;逆境胁迫;全基因组测序;基因功能注释

外文关键词：Wickerhamomyces anomalus;adversity stress;whole-genome sequencing;gene functional annotation

摘要：该研究以一株产酯异常威克汉姆酵母(Wickerhamomyces anomalus)C11为研究对象,利用平板生长法对其逆境胁迫耐受性进行研究。采用Illumina二代测序技术对异常威克汉姆酵母C11的全基因组进行测序,采用直系同源蛋白簇(COG)、基因本体论(GO)、京都基因与基因组百科全书(KEGG)以及碳水化合物活性酶(CAZy)数据库对其功能基因进行注释分析,并基于病原体与宿主的相互作用(PHI)数据库对其致病性进行分析。结果表明,异常威克汉姆酵母C11具有较好的环境耐受性,可耐受100 g/L氯化钠、25 mmol/L过氧化氢、500 mmol/L甘露醇、pH 3.0~10.0、16℃低温、37℃高温以及40 mmol/L铅的胁迫处理。异常威克汉姆酵母C11基因组全长11.48 Mbp,GC含量为34.42%,编码4 249个基因。异常威克汉姆酵母C11基因组从COG、GO和KEGG数据库中分别注释到1 641、3 346和3 145个功能基因。此外,异常威克汉姆酵母C11基因组中含有32个糖苷水解酶相关基因,39个糖基转移酶相关基因,这些功能基因可能与其产酯能力和逆境耐受性相关。毒力基因预测结果鉴定出毒性减弱基因676个,致病力无影响基因391个,致病力丧失基因134个,致死性基因84个,毒力增强(超毒力)基因39个,进一步证实了异常威克汉姆酵母C11毒性较小,安全性较高。
In this study,using an ester-producing strain Wickerhamomyces anomalus C11 as the research object,the stress tolerance of the strain was studied by plate growth method.The whole-genome of W.anomalus C11 was sequenced with Illumina second-generation sequencing technology,and the functional genes were annotated and analyzed using clusters of orthologous groups(COG),gene ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)and carbohydrate active enzymes(CAZy)database.The pathogenicity was analyzed based on the pathogen-host interaction(PHI)database.The results demonstrated that W.anomalus C11 exhibited good environmental tolerance to multiple stress conditions,including 100 g/L NaCl,25 mmol/L H2O2,500 mmol/L mannitol,pH 3.0-10.0,low temperature(16℃),high temperature(37℃),and 40 mmol/L Pb.The W.anomalus C11 genome had a total length of 11.48 Mbp,GC content of 34.42%,and encoded 4249 genes.The W.anomalus C11 genome was annotated to 1641,3346 and 3145 functional genes from the COG,GO and KEGG databases,respectively.Additionally,the genome harbored 32 glycoside hydrolase-related genes and 39 glycosyltransferase-related genes,which could contribute to its ester-producing capacity and stress tolerance.Virulence gene prediction identified 676 virulence-attenuated genes,391 non-virulent genes,134 avirulent genes,84 lethal genes,and 39 hypervirulent genes,further confirming W.anomalus C11 had low virulence and high safety.