文献类型：期刊文献

中文题名：pH和镁离子协同作用对茶树叶绿素合成的影响

英文题名：Effect of synergistic action of pH and magnesium ions on chlorophyll biosynthesis in tea trees

作者：黎楚楚 林长松 欧阳倩 汪营 杨玉萍 杨璇 王丹 徐欢 徐霖

第一作者：黎楚楚

机构：[1]贵阳学院生物与环境工程学院,贵阳550005;[2]贵州理工学院食品药品制造工程学院,贵阳550005

第一机构：贵阳学院生物与环境工程学院,贵阳550005

年份：2024

卷号：37

期号：6

起止页码：1266-1272

中文期刊名：西南农业学报

外文期刊名：Southwest China Journal of Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD_E2023_2024】；

基金：贵州理工学院高层次人才科研启动经费资助项目(XJGC20190922);贵阳学院2021年硕士研究生科研基金项目(GYU-YJS[2021]-81)。

语种：中文

中文关键词：福鼎大白茶;pH;镁离子浓度;叶绿素合成;转录组

外文关键词：Fuding Dabaicha;pH;Magnesium ion concentration;Chlorophyll synthesis;Transcriptome

摘要：【目的】探明土壤pH与镁离子协同作用下茶树叶绿素的合成规律,为茶树栽培及新品种选育提供理论基础。【方法】以福鼎大白茶为研究对象,采用植物组织酶活性试剂盒测定土壤不同pH[pH 4(P_(1))、pH 5(P_(2))]和不同镁离子浓度[0 mg/L(T_(1),CK)、50 mg/L(T_(1))、100 mg/L(T_(2))、150 mg/L(T_(3))]协同作用下茶树叶绿素合成关键酶——镁离子螯合酶、尿卟啉原Ⅲ合酶和5-氨基酮戊酸脱水酶的活性,并采用高通量技术对各处理福鼎大白茶叶片组织进行转录组测序,在此基础上研究土壤pH与镁离子协同作用对茶树叶绿素合成的影响。【结果】土壤pH与镁离子协同作用对茶树叶绿素合成关键酶——镁离子螯合酶、尿卟啉原Ⅲ合酶和5-氨基酮戊酸脱水酶活性的影响存在差异。其中,P_(2)T_(1)的镁离子螯合酶、尿卟啉原Ⅲ合酶及5-氨基酮戊酸脱水酶活性均最高,分别为492.41、336.28和132.24 U/L;GO和KEGG功能富集分析显示,福鼎大白茶叶片组织发育过程中的关键调控基因主要参与碳水化合物代谢、多糖代谢等过程,差异表达基因主要富集在淀粉和蔗糖代谢、二苯乙烯类等途径;KEGG代谢通路光合作用碳固定途径分析结果显示,P_(2)T_(1)能显著提高茶树的碳固定途径,同时对光合作用和糖类积累均有促进作用。【结论】P_(2)T_(1)(pH 5和镁离子浓度50 mg/L)协同作用对茶树叶绿素合成最好,有利于物质的合成和累积,并能提高茶树的产量和品质。
【Objective】The synthesis pattern of tea tree chlorophyll by synergistic effect of soil pH and magnesium ions was explored to provide theoretical basis for the cultivation of tea tree and breeding of new variety.【Method】Taking Fuding Dabaicha as the study object,the plant tissue enzyme activity kits were used to determine the synergistic effects of the soil with different pH[pH 4(P_(1))and pH 5(P_(2))]and different magnesium ion concentrations[0 mg/L(T_(1),CK),50 mg/L(T_(1)),100 mg/L(T_(2))and 150 mg/L(T_(3))]on the key enzymes of chlorophyll synthesis in tea tree,including magnesium chelatase,uroporphyrinogen III synthase and 5-amino ketoglutarate dehydratase.The high-throughput technology was used for transcriptomic sequencing of the leaf slices tissue of Fuding Dabaicha with different treatments,and then the synergistic effect of soil pH and magnesium ion on the synthesis of chlorophyll in tea tree were studied.【Result】The soil pH and magnesium ions had different synergistic effects on the key enzymes of chlorophyll synthesis in tea tree(magnesium chelatase,uroporphyrinogen III synthase and 5-amino ketoglutarate dehydratase).The activities of magnesium chelatase,uroporphyrinogen III synthase and 5-amino ketoglutarate dehydratase treated with P_(2)T_(1)were the highest with 492.41,336.28 and 132.24 U/L,respectively.The analysis of GO and KEGG functional enrichment showed that the key regulatory genes in the development of Fuding Dabaicha leaves were mainly involved in the processes of carbohydrate metabolism and polysaccharide metabolism.The differentially expressed genes were mainly enriched in starch and sucrose metabolism,stilbenes and other pathways.The analysis of KEGG metabolic pathway of photosynthesis of carbon fixation pathway showed that P_(2)T_(1) treatment significantly improved the carbon fixation pathway of tea tree,and had a promotional effect on both photosynthesis and sugar accumulation.【Conclusion】P_(2)T_(1)treatment(the synergistic effects of pH 5 and 50 mg/L magnesium ion)of tea tree has the best effect on chlorophyll synthesis,which is beneficial to the synthesis and accumulation of materials,and is advantageous to the quality and yield of tea tree.