文献类型：期刊文献

英文题名：The Alleviating Effect of Arginine on Ethanol Stress in Wickerhamomyces anomalus

作者：Li, Yinfeng Wang, Yujie Liu, Shuangyan Jiang, Guilan Huang, Mingzheng Liu, Xiaozhu

第一作者：李银凤

通信作者：Huang, MZ[1];Liu, XZ[1]|[14440b58e59f21a634cf7]刘晓柱;[14440edb5c5be18e167dc]刘晓柱;

机构：[1]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China;[2]Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2025

卷号：11

期号：7

外文期刊名：JOURNAL OF FUNGI

收录：;Scopus(收录号：2-s2.0-105011631145);WOS:【SCI-EXPANDED(收录号:WOS:001535426800001)】；

基金：This study was supported by the Guizhou Provincial Science and Technology Foundation (Qiankehejichu MS [2025]192, Qiankehejichu-ZK [2023]yiban 137, Qiankehepingtai KXJZ [2024]021, QKHJC-ZK [2022] YB180).

语种：英文

外文关键词：Wickerhamomyces anomalus; ethanol stress; arginine; transcriptomics; metabolomics; nitric oxide

摘要：Yeast cells are passively exposed to ethanol stress during alcoholic fermentation, ultimately impairing cell viability and reducing fermentation efficiency. Arginine, a versatile amino acid, plays a crucial role in regulating cellular responses to various stresses. This study aimed to explore the underlying mechanism by which arginine protects Wickerhamomyces anomalus against ethanol stress. The effects of arginine supplementation (5 mM) under ethanol stress (9% v/v) on cell survival, reactive oxygen species (ROS) production, cellular and mitochondrial membrane integrity, and nitric oxide synthesis were investigated using fluorescent staining methods. Furthermore, differentially expressed genes (DEGs) and metabolites (DEMs) were identified through transcriptomics and metabolomics analyses. The results demonstrated that exogenous arginine enhanced cell survival, reduced ROS levels, maintained cellular and mitochondrial membrane integrity, stimulated nitric oxide production, and modulated gene expression and metabolic pathways involved in pyruvate metabolism, yeast meiosis, fatty acid degradation, glycerophospholipid metabolism, and the biosynthesis of various secondary metabolites. These findings provide intriguing insights into the mechanistic role of arginine in enhancing the tolerance of W. anomalus to ethanol stress, and broaden its application in the fermentation industry for alcoholic beverages.