文献类型：期刊文献

英文题名：Hybridization chain reaction and DNAzyme-based dual signal amplification strategy for sensitive fluorescent sensing of aflatoxin B1 by using the pivot of triplex DNA

作者：Zou, Li Zhang, Manjun Li, Mengyan Xiao, Zhiyou Ling, Liansheng

第一作者：Zou, Li

通信作者：Zou, L[1]

机构：[1]Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Peoples R China;[2]Sun Yat Sen Univ, Sch Chem, Guangzhou 510275, Peoples R China;[3]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China

第一机构：Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Peoples R China

通信机构：corresponding author), Guangdong Pharmaceut Univ, Sch Pharm, Guangzhou 510006, Peoples R China.

年份：2022

卷号：158

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：;EI(收录号：20222812357305);Scopus(收录号：2-s2.0-85133867479);WOS:【SCI-EXPANDED(收录号:WOS:000886092600006)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 81903570, 21375153 and 21768002) .

语种：英文

外文关键词：Aptasensor; Aflatoxin B1; Hybridization chain reaction; DNAzyme; Triplex DNA

摘要：This work developed an enzyme-free fluorescent aptasensor for sensitive aflatoxin B1 (AFB1) detection based on a dual signal amplification strategy of hybridization chain reaction (HCR) and Zn2+-dependent DNAzyme. In the presence of AFB1, the aptamer specifically binds to the target, releasing the blocking DNA, which can initiate HCR between hairpin probes H1 and H2. With the addition of the substrate strand (Zn-Sub) and enzyme strand (Zn-Enz) of DNAzyme, HCR product can hybridize with Zn-Sub and Zn-Enz to form triplex DNA and Y-shaped structure together, which further activates the DNAzyme to cleave Zn-Sub. Then, two separated fragments of ZnSub respectively hybridize with the fluorescent probe and quencher probe, which results in a dramatic increase in the fluorescence intensity. The proposed aptasensor shows high sensitivity and selectivity for AFB1 detection with a detection limit of 0.22 nmol/L in a linear range of 0.4-16 nmol/L. Moreover, the fluorescent aptasensor exhibits acceptable applicability for detecting AFB1 in oil samples with satisfactory recoveries of 92.2-107.8%. Results are also in agreement with those of the ELISA method, indicating that the fluorescent sensing strategy has great potential applications in food safety control.