文献类型：期刊文献

英文题名：A Novel "Turn-On" Fluorescent Sensor for Screening Triplex DNA Binder Based upon Molecular Beacon

作者：Fu, Yuanxiang Si, Hengdan Chen, Juan Zhang, Wenjuan Feng, Shuang Xiao, Zhiyou

第一作者：符远翔

通信作者：Xiao, ZY[1]|[14440ef2cf80e422d3de7]肖志友;

机构：[1]Guizhou Inst Technol, Sch Chem Engn, 1st Caiguan Rd, Guiyang 550003, Peoples R China

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Chem Engn, 1st Caiguan Rd, Guiyang 550003, Peoples R China.|贵州理工学院化学工程学院;贵州理工学院;

年份：2022

卷号：7

期号：42

外文期刊名：CHEMISTRYSELECT

收录：;Scopus(收录号：2-s2.0-85141961274);WOS:【SCI-EXPANDED(收录号:WOS:000879803100001)】；

基金：The study was supported by the National Natural Science Foundation of China (No. 21768002), Science and Technology Foundation of Guizhou Province (Qiankehe Jichu ZK[2021]077), Scientific Research Foundation for Advanced Talents of Guizhou Institute of Technology (XJGC20190628).

语种：英文

外文关键词：fluorescent sensors; molecular beacons; screening; triplex DNA binders; turn-on

摘要：Triplex DNA plays an essential role in molecular recognition, gene regulation, diagnosis, and treatment of genetic diseases. However, triplex DNA has some shortcomings, such as poor internal Hoogsteen hydrogen bond stability and the difficult formation of parallel triplex DNA under physiological conditions, limiting its applications to a certain extent. Therefore, it is crucial to screen intercalators to enhance triplex DNA stability. Herein, we established a novel "turn-on" fluorescence sensor for screening triplex DNA binders based upon molecular beacon. Eight different organic small molecules were screened. The fluorescence of the sensor was significantly enhanced in the presence of berberine, coralyne, palmatine, and neomycin. These four small molecules affect stabilizing triplex DNA as an intercalator; the other four small molecules, kanamycin, tetracycline, ethidium bromide, and 4 ', 6-diamidino-2-phenylindole did not show any noticeable intercalation effect. The results of circular dichroism and melting curve were consistent with those of the fluorescence spectrum.