文献类型：期刊文献

英文题名：Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment

作者：Guo, Dong-Gui Zhu, Jun Wang, Hui-Juan Pan, Bo-Wen

第一作者：郭东贵

通信作者：Guo, DG[1]

机构：[1]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550025, Peoples R China;[2]Guizhou Univ, Natl & Local Joint Engn Res Ctr Exploit Homol Reso, Guiyang 550025, Peoples R China;[3]Guizhou Univ Tradit Chinese Med, Coll Pharm, Guiyang 550025, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550025, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2024

卷号：21

期号：5

外文期刊名：CHEMISTRY & BIODIVERSITY

收录：;Scopus(收录号：2-s2.0-85189905867);WOS:【SCI-EXPANDED(收录号:WOS:001199221400001)】；

基金：This research was financially supported by Guizhou Provincial Science and Technology Projects (Qian Ke He Ji Chu - ZK [2021] General 520, Qian Ke He Ji Chu - ZK [2022] General 173); Guizhou Province Projects (Qian Jiao He KY Zi [2022]253); Guizhou University Cultivation Project (Gui Da Cultivation [2020] No. 52).

语种：英文

外文关键词：Cyclomorusin; High glucose concentrations; Osteoclasts; RANKL/RANK/TRAF6 pathway

摘要：Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory process. In this study, we used a co-culture system of RAW264.7 and MC3T3-E1 cells under HG concentration to explore the effect of CYM on OCs in a HG environment. The effects of CYM on the formation and function of OCs were observed using TRAP-positive cell counts and bone resorption pits. Then, mRNA and protein expression levels of OCs-related genes were detected by real-time qPCR and western blotting. The results showed that CYM had an inhibitory effect on OCs differentiation and bone resorption, reduced mRNAs expression of OCs-associated genes, and downregulated RANKL/RANK/TRAF6 pathway that mediates OCs differentiation. CYM could be a promising natural compound against diabetic osteoporosis. image