文献类型：期刊文献

英文题名：Novel Method for the Quantitative Analysis of Protease Activity: The Casein Plate Method and Its Applications

作者：Zhang, Xin Shuai, Yao Tao, Han Li, Cuiqin He, Laping

第一作者：Zhang, Xin;张璇

通信作者：Li, CQ[1];He, LP[1];He, LP[2];Li, CQ[3]

机构：[1]Guizhou Univ, Key Lab Agr & Anim Prod Store & Proc Guizhou Prov, Guiyang 550025, Peoples R China;[2]Guizhou Inst Technol, Coll Artificial Intelligence & Elect Engn, Guiyang 550005, Peoples R China;[3]Guizhou Univ, Coll Liquor & Food Engn, Guiyang 550025, Peoples R China;[4]Guizhou Univ, Sch Chem & Chem Engn, Guiyang 550025, Peoples R China

第一机构：Guizhou Univ, Key Lab Agr & Anim Prod Store & Proc Guizhou Prov, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Key Lab Agr & Anim Prod Store & Proc Guizhou Prov, Guiyang 550025, Peoples R China;corresponding author), Guizhou Univ, Coll Liquor & Food Engn, Guiyang 550025, Peoples R China;corresponding author), Guizhou Univ, Sch Chem & Chem Engn, Guiyang 550025, Peoples R China.

年份：2021

卷号：6

期号：5

起止页码：3675-3680

外文期刊名：ACS OMEGA

收录：;Scopus(收录号：2-s2.0-85100793377);WOS:【SCI-EXPANDED(收录号:WOS:000618860000022)】；

基金：This work was financially supported by the Key Agricultural Project of Guizhou Province (QKHZC-[2019]2382 and QKHZC-[2016]2580), the National Natural Science Foundation of China (31870002 and 31660010), the Guizhou Province Science and Technology Plan Project (Qiankehe talents project [2018]5781, [2017]5788-11, and [2017]5788), and the Research Project of Guizhou University ((2019)33).

语种：英文

摘要：No simple methods are used for the quantitative analysis of the protease activity in colored food up till now. Thus, this study aims to establish a new and simple method for the quantitative detection of protease activity, especially in colored food. The detection accuracy, detection limit, and repeatability of the casein plate method were analyzed. Then, the application of the casein plate method in sample detection and recovery was further evaluated. The results showed that the casein plate method for the quantitative detection of protease activity has high accuracy, high precision, and low detection limit. The recoveries of eight kinds of colored samples were in the range of 92.26-97.84%, and the relative standard deviation (RSD) was in the range of 3.56-10.88%. The results of the casein plate method exhibited high accuracy. This indicated that the method was suitable for the detection of colored samples. The casein plate method for the quantitative detection of protease activity is simple. The newly constructed casein plate method has broad potential application value in food industry, especially for the detection of dark food.