文献类型：期刊文献

英文题名：Activation of the Unfolded Protein Response via Co-expression of the HAC1i Gene Enhances Expression of Recombinant Elastase in Pichia pastoris

作者：Han, Minghai Wang, Weixian Zhou, Jianli Gong, Xun Xu, Cunbin Li, Yinfeng Li, Qiang

第一作者：韩铭海

通信作者：Han, Minghai|[144402eaf094eaf27b6d2]韩铭海;

机构：[1] College of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyan, 550000, China; [2] Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou, 510006, China; [3] School of Biotechnology, Jiangnan University, Wuxi, 214122, China

第一机构：贵州理工学院

通信机构：College of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyan, 550000, China|贵州理工学院;

年份：2020

卷号：25

期号：2

起止页码：302-307

外文期刊名：Biotechnology and Bioprocess Engineering

收录：EI(收录号：20201708509144);Scopus(收录号：2-s2.0-85083420597)

语种：英文

外文关键词：Chemical activation - Yeast - Recombinant proteins - Methanol

摘要：The effects of activation of the unfolded protein response (UPR) via co-expression of the HAC1i gene on the production of the recombinant Pseudomonas aeruginosa elastase (rPAE) in Pichia pastoris GS115 were evaluated in this study. The results showed that expression of the HAC1i gene significantly increased the level of Kar2p (a hallmark of UPR activation) in P. pastoris GS115, demonstrating activation of the UPR. This gene did not affect the growth of yeast in the buffered glycerol-complex medium but stimulated its growth in the buffered methanolcomplex medium. Co-expression of the HAC1i gene enhanced the expression level of the heterogeneously Nglycosylated forms of rPAE, as the caseinolytic activity in the supernatant of the various glycoforms of rPAE expressed in P. pastoris GS115/HAC1 was increased 1.8—3.9-fold compared to that in the control strain P. pastoris GS115, respectively. The stimulating effects of co-expression of the gene on rPAE production were observed when 0.5, 1.0, and 2.0% methanol were added every 24 h, as the caseinolytic activity of supernatants of P. pastoris GS115/HAC1 expressing wild-type of rPAE was increased 3.3-, 1.9-, and 1.7-fold at the corresponding methanol concentration. Further, activation of UPR via co-expression of the HAC1i gene enhanced rPAE secretion in P. pastoris at 20, 24, and 28°C, as the caseinolytic activity of supernatants of P. pastoris GS115/HAC1 expressing wild-type rPAE was increased 2.3-, 2.1-, and 2.8-fold over the tested temperatures. ? 2020, The Korean Society for Biotechnology and Bioengineering and Springer.