文献类型：期刊文献

英文题名：Hydrolyzing Laminaria japonica with a combination of microbial alginate lyase and cellulase

作者：Sun, Chixiang Zhou, Jianli Duan, Guoliang Yu, Xiaobin

第一作者：Sun, Chixiang

通信作者：Yu, XB[1]

机构：[1]Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Sch Biotechnol, Minist Educ, 1800 Li Hu Rd, Wuxi 214122, Jiangsu, Peoples R China;[2]Guizhou Inst Technol, Sch Food & Drug Mfg Engn, 1 Caiguan Rd, Guiyang 550003, Peoples R China

第一机构：Jiangnan Univ, Key Lab Carbohydrate Chem & Biotechnol, Sch Biotechnol, Minist Educ, 1800 Li Hu Rd, Wuxi 214122, Jiangsu, Peoples R China

通信机构：corresponding author), Jiangnan Univ, Sch Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China.

年份：2020

卷号：311

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000539210700013)】；

基金：This study was supported by the national first-class discipline program of Light Industry Technology and Engineering (LITE2018-11), and the Open Project of Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of Education (Grant No. KLCCB-KF201808). The authors would like to thank the Centre of Analysis and Testing of the Jiangnan University, the State Key Laboratory of Food Science and Technology, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences for their expert technical assistance.

语种：英文

外文关键词：Laminaria japonica; Alginate lyase; Cellulase; Alginate oligosaccharide; Fertilizer

摘要：A novel seaweed-hydrolyzing strain designated as Pseudoalteromonas sp. Alg6B was isolated from the surface of brown seaweed (Laminaria japonica). The activity of crude alginate lyase produced by Alg6B was 54.5 U/ml and the main products of hydrolyzing alginate were disaccharide and tetrasaccharide. The hydrolysis rate of seaweed reached up to 97% after combining 3% (v/v) Alg6B and 0.2% (w/v) solid cellulase. In kelp hydrolysate, the contents of nutrients are much more than raw seaweed. Alg6B grows quickly and has the ability of producing alginate oligosaccharides with low molecular weight (MW) (<= 2 kDa). Furthermore, this study demonstrates that a combination of microbial alginate lyase and cellulase could almost hydrolyze seaweed completely. This research indicated that Alg6B could provide a feasible pathway to produce alginate oligosaccharides (AOS), and the synergistic effect of alginate lyase and cellulase on seaweed bioconversion can potentially pave the way to the sustainable production of seaweed fertilizer.