文献类型：期刊文献

中文题名：天麻素脂质体的制备及其结构表征

英文题名：Preparation and structure characterization of gastrodine liposomes

作者：覃引 邱树毅 张振 周剑丽 胡女丹

第一作者：覃引

机构：[1]贵州理工学院制药工程学院;[2]贵州省发酵工程与生物制药重点实验室

第一机构：贵州理工学院食品药品制造工程学院

年份：2015

卷号：0

期号：5

起止页码：29-32

中文期刊名：中国生化药物杂志

外文期刊名：Chinese Journal of Biochemical Pharmaceutics

基金：贵州理工学院科研基金(XJ2K20131204)

语种：中文

中文关键词：天麻素;脂质体;包封率;制备工艺;正交试验

外文关键词：gastrodine;liposome;entrapment efficiency;preparation process;orthogonal test

摘要：目的研究天麻素脂质体的最佳制备工艺,并表征其结构。方法采用薄膜—超声法制备。以包封率为指标,卵磷脂与胆固醇之间的质量比、油水相的体积比和超声时间为考察因素,采用L9（3^4）正交试验法优化制备工艺;显微镜下观察脂质体的形态、粒径大小和分布,并测定其包封率和稳定性。结果回归方程式为Y=239.75X＋0.0207（n=5）,R^2=0.9996,在0.002-0.010 mg/m L范围内具有良好的线性关系。最佳工艺为：卵磷脂与胆固醇的质量比为4∶1、水油相的体积比为3∶1、超声时间控制为60 min;脂质体呈现类球形或椭圆形,形态规则、大小均匀;平均包封率为60.65%,平均粒径分布为327 nm,稳定性良好。结论优选后的制备工艺稳定,所需设备简单,包封率较高,粒径较均匀,可适用于包埋水溶性药物。
Objective To study the optimum preparation process of gastrodin liposome, characterization its structure.Methods Perpared gastrodin liposome with film-ultrasonic method.The entrapment efficiency as index, quality between lecithin and cholesterol ratio, oil-water phase volume ratio and ultrasonic time as factors by L9 （3^4 ） orthogonal test to optimize the preparation process.Observed the liposome morphology, particle size and distribution under the microscope, and determined the entrapment efficiency and stability.Results Regression equation was Y=239.75 ＋0.0207X（n=5）, R^2 =0.9996, 0.002 to 0.010 mg/mL had a good linear relationship.The optimum of preparation process： lecithin and cholesterol quality ratio was 4：1, water and oil phase volume ratio of 3：1, ultrasonic time control for 60 min;Liposomes showed spherical or oval shape, regular shape, uniform size.The average entrapment efficiency was 60.65%, the average particle size distribution at 327 nm, and it had good stability.Conclusion The preparation of gastrodine liposome has advantages of simple, higher entrapment efficiency and even particle size.The method can be used for embedded water-soluble medicine.