文献类型：期刊文献

中文题名：应用超高效液相色谱-飞行时间质谱鉴定大口黑鲈体内鞣花酸代谢物

英文题名：Identification of Ellagic Acid Metabolites in Largemouth Bass(Micropterus salmoides)Using Ultra-high-performance Liquid Chromatography-time-of-flight Mass Spectrometry

作者：朱元馨 刘怡君 李海飞 吴念蓉 罗志军 周贤君 罗波 张勇民

第一作者：朱元馨

机构：[1]贵州中医药大学药学院,贵州贵阳550025;[2]贵阳倍隆生物科技有限公司,贵州贵阳550201;[3]贵州大学动物科学学院,贵州贵阳550025;[4]贵州理工学院食品与制药工程学院,贵州贵阳550025;[5]巴黎索邦大学分子化学研究所,法国巴黎75252

第一机构：贵州中医药大学药学院,贵州贵阳550025

年份：2026

卷号：55

期号：5

起止页码：104-112

中文期刊名：山东化工

外文期刊名：Shandong Chemical Industry

基金：中国药用植物药效与利用省部共建国家重点实验室(原中国贵州医科大学药用植物药效与利用国家重点实验室)开放课题资助(合同编号:FAMP202106K)。

语种：中文

中文关键词：鞣花酸;超高效液相色谱-四极杆飞行时间质谱;代谢物;大口黑鲈鱼

外文关键词：ellagic acid;ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry;metabolites;largemouth bass(Micropterus salmoides)

摘要：针对复杂生物基质中鞣花酸(Ellagic acid,EA)及其痕量、易氧化代谢物的系统鉴定难题,本研究建立了一种基于超高效液相色谱-四极杆飞行时间质谱(UHPLC-Q-TOF MS)的分析方法。通过优化样品前处理流程,引入维生素C与乙二胺四乙酸(EDTA)联合保护策略,有效抑制了多酚类化合物的氧化降解。结合高分辨率质谱与数据库匹配,实现了对结构相似代谢物的初步识别。将该方法应用于摄食含0.04%鞣花酸饲料的大口黑鲈后,在其血液样本中推测鉴定出Luteic acid、黄质鞣花酸、尿石素C、尿石素D、尿石素E、尿石素M5、尿石素M6、尿石素M7及新发现代谢物TP11共9种代谢产物;而在肝脏与肠道组织中未检出相关代谢物,表明该方法对血液基质具有更佳的分析适用性。本研究建立的方法稳定、灵敏,适用于复杂生物样品中多酚类代谢物的非靶向筛查与鉴定,为水产动物植物活性成分的代谢研究提供了可行的分析手段。
To address the systematic identification challenge of ellagic acid(EA)and its trace,oxidizable metabolites in complex biological matrices,this study established an analytical method based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF MS).By optimizing the sample preparation process and introducing a combined protective strategy using vitamin C and EDTA,the oxidative degradation of polyphenolic compounds was effectively suppressed.The preliminary identification of structurally similar metabolites was achieved by combining high-resolution mass spectrometry with database matching.Applying this method to largemouth bass(Micropterus salmoides)fed a diet containing 0.04%EA led to the tentative identification of nine metabolites in blood samples,including luteic acid,flavellagic acid,urolithins C,urolithins D,urolithins E,urolithins M5,urolithins M6,urolithins M7,and a newly discovered metabolite TP11.In contrast,no relevant metabolites were detected in liver or intestinal tissues,indicating better analytical applicability of this method for blood matrices.The established method is stable and sensitive,suitable for the non-targeted screening and identification of polyphenolic metabolites in complex biological samples,providing a feasible analytical approach for studying the metabolism of plant-derived active components in aquatic animals.