文献类型：期刊文献

英文题名：Crude Oil Degrading Fingerprint and the Overexpression of Oxidase and Invasive Genes for n-hexadecane and Crude Oil Degradation in the Acinetobacter pittii H9-3 Strain

作者：Wang, Yang Wang, Qiuyu Liu, Limei

第一作者：Wang, Yang

通信作者：Wang, Y[1]

机构：[1]Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150025, Heilongjiang, Peoples R China;[2]Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Heilongjiang, Peoples R China;[3]Guizhou Inst Technol, Sch Resources & Environm Engn, Guiyang 550003, Guizhou, Peoples R China

第一机构：Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150025, Heilongjiang, Peoples R China

通信机构：corresponding author), Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150025, Heilongjiang, Peoples R China.

年份：2019

卷号：16

期号：2

外文期刊名：INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH

收录：;WOS:【SSCI(收录号:WOS:000459112100023)，SCI-EXPANDED(收录号:WOS:000459112100023)】；

基金：The study was supported by the Doctoral Initial Fundation of Harbin Normal University and the State Bureau of Forestry 948 project (2008-4-34).

语种：英文

外文关键词：crude oil; GC-MS; fingerprints; biodegradation; Acinetobacter pittii

摘要：A crude oil-degrading bacterium named strain H9-3 was isolated from crude oil contaminated soil in the Northeastern area of China. Based on its morphological characteristics and 16S rDNA sequence analysis, strain H9-3 is affiliated to Acinetobacter pittii in the group of Gammaproteobacteria. The strain was efficient in removing 36.8% of the initial 10 gL-1 of crude oil within 21 days. GC-MS was performed and a preference was shown for n-C10, n-C11, i-C14, i-C17, i-C34, n-C12, n-C13, n-C14, n-C27, n-C32 and i-C13, over n-C16, n-C18-C22, n-C24-n-C31, and n-C36. This can be regarded as the specific fingerprint for crude oil degradation by strain H9-3 of Acinetobacter pittii. In addition to crude oil, it was shown that soybean oil and phenols can be utilized as carbon sources by strain H9-3. It was also shown that aniline and -naphthol cannot be utilized for growth, but they can be tolerated by strain H9-3. Methylbenzene was neither utilized nor tolerated by strain H9-3. Although n-hexadecane was not preferentially consumed by strain H9-3, during culture with crude oil, it could be utilized for growth when it is the sole carbon source. The degradation of some branched alkanes (i-C14, i-C17 and i-C34) and the preferential degradation of crude oil over phenols could be used as a reference for distinguishing A. pittii from A. calcoaceticus. The difference in gene expression was very significant and was induced by diverse carbon sources, as shown in the qRT-PCR results. The oxidation and adhesion events occurred at high frequency during alkane degration by Acinetobacter pittii strain H9-3 cells.