文献类型：期刊文献

英文题名：Distribution Characteristics of Nitrogen-Removing Bacteria in the Rhizosphere of Wetland Plants in Urban Rivers of the Karst Region in Southwest China

作者：Yin, Xingjia Yao, Liangzhu Li, Yi

第一作者：Yin, Xingjia

通信作者：Li, Y[1]

机构：[1]Sun Yat Sen Univ, Sch Marine Sci, Zhuhai 519082, Peoples R China;[2]Hubei Engn Univ, Coll Life Sci & Technol, Xiaogan 432000, Peoples R China;[3]Power China Guiyang Engn Corp Ltd, Guiyang 550081, Peoples R China;[4]Guizhou Inst Technol, Sch Resource & Environm Engn, Guiyang 550001, Peoples R China

第一机构：Sun Yat Sen Univ, Sch Marine Sci, Zhuhai 519082, Peoples R China

通信机构：corresponding author), Guizhou Inst Technol, Sch Resource & Environm Engn, Guiyang 550001, Peoples R China.|贵州理工学院;

年份：2025

卷号：45

期号：6

外文期刊名：WETLANDS

收录：;Scopus(收录号：2-s2.0-105008296506);WOS:【SCI-EXPANDED(收录号:WOS:001510504300003)】；

基金：This work was supported the Science and Technology Fund of Guizhou Province (Guizhou Science and Technology Foundation [2020]1Y184), the Youth Science and Technology Talent Development Project of Guizhou Education Department (Guizhou Science and Technology Cooperation Foundation [2018]254) and the Scientific Research Program of Hubei Provincial Department of Education (Q20212701).

语种：英文

外文关键词：Karst region; Urban river wetland; Wetland plants; nirS-type denitrifiers; Anammox bacteria

摘要：Microorganisms are crucial for nitrogen removal processes in river ecosystems, particularly in the rhizosphere of plants where microbial activity is intense. This study investigates nitrogen-removing microbial communities in the rhizosphere sediments of wetland plants in the Xiaoche River urban wetland of Guiyang. High-throughput sequencing was employed to analyze the bacterial diversity in the rhizosphere and non-rhizosphere sediments of Acorus calamus, Cyperus alternifolius, and Echinochloa crus-galli. Additionally, the abundance of nirS-type denitrifiers and anammox bacteria in the sediments was determined using real-time quantitative PCR. Sequencing results indicated 16 bacterial phyla with over 1% relative abundance in both rhizosphere and non-rhizosphere sediments, dominated by Proteobacteria, Bacteroidetes, and Acidobacteria, collectively accounting for over 50% of the relative abundance. Proteobacteria were more abundant in rhizosphere, while Bacteroidetes were more prevalent in the non-rhizosphere. There were 24 bacterial genera with relative abundance greater than 1%, and the dominant genera varied significantly among different sampling sites. Cluster analysis revealed significant differences at genus-level between rhizosphere and non-rhizosphere, with high similarity between the populations of Acorus calamus and Cyperus alternifolius. Quantitative gene results indicated that the abundance of nirS and anammox 16S rRNA genes was lower in the non-rhizosphere than in the rhizospheres with nirS and anammox 16S rRNA gene abundance levels reaching 10(10) copies g(- 1) dry sediment, suggesting a high richness of nitrogen-removing bacteria in the plants rhizospheres. Redundancy analysis (RDA) showed that the environmental factors most influencing the abundance of these two genes were total phosphorus (TP), organic matter (OM), and ammonium nitrogen (NH4+-N).