文献类型：期刊文献

英文题名：Chemical Composition, and Antioxidant and Cholinesterase Inhibitory Activities of Lindera glauca Fruit Essential Oil and Molecular Docking Studies of Six Selected Compounds

作者：Sun, Zhenchun Su, Xiankun Lin, Yechun Long, Chongyan Zhang, Yazhou Zhao, Tianming

第一作者：Sun, Zhenchun

通信作者：Zhao, TM[1]

机构：[1]Guizhou Acad Tobacco Sci, Guiyang 550081, Peoples R China;[2]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China;[3]Guizhou Univ Tradit Chinese Med, Sch Pharm, Guiyang 550025, Peoples R China

第一机构：Guizhou Acad Tobacco Sci, Guiyang 550081, Peoples R China

通信机构：corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2023

卷号：9

期号：2

外文期刊名：HORTICULTURAE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000945936700001)】；

基金：This research was funded by the Science and Technology Project of Guizhou Company of China Tobacco Corporation (2022XM14), the Science and Technology Program of Guizhou Province (No. Qian Ke He [2018]1071), the High-level Talent Research Funding Project of Guizhou Institute of Technology (XJGC20161206) and the College Students' Innovative Training Project (S202114440094).

语种：英文

外文关键词：antioxidant; beta-caryophyllene; cholinesterase inhibitor; essential oil; geranyl acetate; limonene; Lindera glauca; molecular docking

摘要：Lindera glauca is a shrub or small tree mostly distributed in China, Japan and Korea. However, reports on the biological activities of Lindera glauca fruit essential oil (LGFEO) are limited. The study on its chemical composition, and antioxidant and cholinesterase inhibitory activities were performed, along with molecular docking of six selected compounds. The LGFEO was extracted by hydro distillation and analyzed by GC-MS and GC-FID. Antioxidant activities of LGFEO were evaluated by three methods with different mechanisms. Acetylcholinesterase and butyrylcholinesterase inhibitory activities of LGFEO were tested. A total of 48 components were identified representing 95.74% of the total composition of LGFEO in which the major compounds were (E)-beta-ocimene (41.53%), alpha-copaene (13.17%), delta-cadinene (6.20%), 3-carene (5.89%) and eucalyptol (3.57%). Weak antioxidant activities of LGFEO in three assays (9.52, 11.36 and 38.98 mu mol TE/g, respectively) were observed. LGFEO showed obvious cholinesterase inhibitory activities at the final concentrations of 50 and 20 mu g/mL. IC50 values for acetylcholinesterase and butyrylcholinesterase were 46.48 and 34.85 mu g/mL, respectively. Molecular docking revealed that geranyl acetate, beta-caryophyllene and limonene had lower binding affinities in the range of -7.1 to -6.1 kcal/mol through hydrophobic interactions and hydrogen bond. Six compounds including 3-carene, limonene, eucalyptol, (E)-beta-ocimene, geranyl acetate and beta-caryophyllene could contribute together to cholinesterase inhibitory activities of LGFEO. This essential oil indicated low potential as natural antioxidant, but it could be potentially used as cholinesterase inhibitor with possible application in food, aromatherapy and pharmaceutical industries.