文献类型：期刊文献

英文题名：Synergistic effects of bloom helicase (BLM) inhibitor AO/854 with cisplatin in prostate cancer

作者：Ma, Xiaoyan Tian, Fu Xiao, Yuanpin Huang, Mengqiu Song, Dandan Chen, Xinlin Xu, Houqiang

第一作者：马小彦;Ma, Xiaoyan

通信作者：Xu, HQ[1];Xu, HQ[2]

机构：[1]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550003, Peoples R China;[2]Guizhou Univ, Key Lab Anim Genet, Breeding & Reprod Plateau Mountainous Reg, Minist Educ,Coll Life Sci, Guiyang 550025, Peoples R China;[3]Guizhou Univ, Coll Anim Sci, Guiyang 550025, Peoples R China;[4]Moutai Inst, Dept Brewing Engn, Renhuai 564500, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Univ, Key Lab Anim Genet, Breeding & Reprod Plateau Mountainous Reg, Minist Educ,Coll Life Sci, Guiyang 550025, Peoples R China;corresponding author), Guizhou Univ, Coll Anim Sci, Guiyang 550025, Peoples R China.

年份：2024

卷号：14

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-85207209744);WOS:【SCI-EXPANDED(收录号:WOS:001340423800004)】；

基金：This work was supported by Guizhou Institute of Technology High-level Talent Scientific Research Start-up Fund, grant number 2023GCC066. This work was also supported by National Natural Science Foundation of China, grant number 31860242. This research was also funded by Zunyi Technology and Big data Bureau Moutai institute Joint Science and Technology Research and Development Project, grant number ZunaShiJiaoHe HZ zi[2020]316.

语种：英文

外文关键词：BLM; AO/854; CDDP; Combined therapy

摘要：To determine the synergistic effect and mechanism of AO/854, a new Bloom syndrome protein (BLM) helicase inhibitor, and cisplatin (CDDP), a DNA-crosslinking agent, cell viability assays, neutral comet assays, and Western blotting (WB) were performed on prostate cancer (PCa) cells. According to our findings, combining AO/854 and CDDP enhanced the antiproliferative capabilities of PC3 cell lines. As evidenced by the upregulation of gamma H2AX, cleaved caspase-3/caspase-3, and BAX/Bcl-2, AO/854 dramatically increased PC3 apoptosis and DNA damage induced by CDDP. Furthermore, combining AO/854 and CDDP synergistically inhibited PC3 cell migration and invasion. In addition, AO/854 inhibited CDDP-induced S-phase cell-cycle arrest in PC3 cells while enhancing G2/M-phase cell-cycle arrest. In vivo, the antitumor efficacy of the combination therapy group was greater than that of the groups treated with AO/854 or CDDP alone. Our findings indicate that synergistic chemotherapy with AO/854 and CDDP may be a novel anticancer strategy for PCa.