文献类型：期刊文献

英文题名：HPLC-ESI-qTOF-MS/MS Characterization, Antioxidant Activities and Inhibitory Ability of Digestive Enzymes with Molecular Docking Analysis of Various Parts of Raspberry (Rubus ideaus L.)

作者：Wu, Lingfeng Liu, Yufeng Qin, Yin Wang, Lu Wu, Zhenqiang

第一作者：Wu, Lingfeng

通信作者：Wang, L[1];Wu, ZQ[2];Wu, ZQ[3]

机构：[1]Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Hainan, Peoples R China;[2]South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China;[3]Sch Pharmaceut Engn, Guizhou Inst Technol, Guiyang 550003, Guizhou, Peoples R China;[4]Pan Asia Jiangmen Inst Biol Engn & Hlth, Jiangmen 529080, Peoples R China

第一机构：Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Hainan, Peoples R China

通信机构：corresponding author), Hainan Univ, Coll Food Sci & Engn, Haikou 570228, Hainan, Peoples R China;corresponding author), South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Guangdong, Peoples R China;corresponding author), Pan Asia Jiangmen Inst Biol Engn & Hlth, Jiangmen 529080, Peoples R China.

年份：2019

卷号：8

期号：8

外文期刊名：ANTIOXIDANTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000482958900038)】；

基金：This work was supported by the Scientific Research Foundation of Hainan University (No. KYQD1901) and Guangdong special funds for science and technology innovation strategy, China (JK2018-352-0203).

语种：英文

外文关键词：Rubus idaeus L; phenolic compounds; HPLC-ESI-HR-qTOF-MS; MS; antioxidant activities; digestive enzymes inhibitors; molecular docking analysis

摘要：The anti-oxidative phenolic compounds in plant extracts possess multiple pharmacological functions. However, the phenolic characterization and in vitro bio-activities in various parts of raspberry (Rubus idaeus L.) have not been investigated systematically. In the present study, the phenolic profiles of leaves (LE), fruit pulp (FPE), and seed extracts (SE) in raspberry were analyzed by HR-HPLC-ESI-qTOF-MS/MS method, and their antioxidant activities and digestive enzymes inhibitory abilities were also investigated. The molecular docking analysis was used to delineate their inhibition mechanisms toward type II diabetes related digestive enzymes. Regardless of LE, FPE, or SE, 50% methanol was the best solvent for extracting high contents of phenolic compounds, followed by 50% ethanol and 100% methanol. The LE of raspberry displayed the highest total phenolic content (TPC) and total flavonoid content (TFC). A total of nineteen phenolic compounds were identified. The quantitative results showed that gallic acid, ellagic acid, and procyanidin C3 were the major constituents in the three extracts. The various parts extracts of raspberry all exhibited the strong antioxidant activities, especially for LE. Moreover, the powerful inhibitory effects of the three extracts against digestive enzymes (alpha-glucosidase and alpha-amylase) were observed. The major phenolic compounds of the three extracts also showed good inhibitory activities of digestive enzyme in a dose-dependent manner. The underlying inhibitory mechanisms of the main phenolic compounds against digestive enzymes were clarified by molecular docking analysis. The present study demonstrated that the various parts of raspberry had strong antioxidant activities and inhibitory effects on digestive enzymes, and can potentially prevent oxidative damage or diabetes-related problems.