文献类型：期刊文献

英文题名：Trehalose-mediated reshaping of the rhizosphere microbiome drives tea root rot progression

作者：Zhu, Qiang Chen, Bowen Hu, Weiting Huang, Yingbo Wang, Shengyuan Feng, Mei Zhao, Jie Yu, Mingyi Li, Mingzhu Gong, Xuejiao

第一作者：朱强;Zhu, Qiang

通信作者：Gong, XJ[1]

机构：[1]Sichuan Acad Agr Sci, Tea Res Inst, Chengdu, Peoples R China;[2]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang, Guizhou, Peoples R China

第一机构：Sichuan Acad Agr Sci, Tea Res Inst, Chengdu, Peoples R China

通信机构：corresponding author), Sichuan Acad Agr Sci, Tea Res Inst, Chengdu, Peoples R China.

年份：2026

卷号：17

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-105031934120);WOS:【SCI-EXPANDED(收录号:WOS:001706818700001)】；

基金：The author(s) declared that financial support was received for this work and/or its publication. This work was supported by the Sichuan Provincial Natural Science Foundation (2024NSFSC1311), Guizhou Provincial Science and Technology Foundation (MS [2025]197), "5 + 1" Agricultural Frontier Technology Research Program of Sichuan Academy of Agricultural Sciences (5 + 1QYGG001), Guizhou Institute of Technology Innovation Exploration Project (no. 2024XSXM009).

语种：英文

外文关键词：biocontrol; rhizosphere microbial community; root exudates; tea root rot disease; trehalose

摘要：Tea (Camellia sinensis [L.] Kuntze) is one of the most economically important crops and as a traditional medicinal plant in the world. The long-term continuous cropping and inappropriate management have led to frequent outbreaks of soil-borne diseases such as root rot, which pose a serious threat to the sustainable development of the tea industry. However, the pathogenesis of tea root rot remains poorly understood. In this study, two novel pathogen fungi, Paraconiothyrium cyclothyrioides F8 and Apiotrichum sporotrichoides F17, were isolated and identified from diseased tea roots. Microbiome analysis revealed significant restructuring of the rhizosphere microbial community in diseased tea plants, with a significant reduction in the abundance of Basidiomycota and marked enrichment of pathogen such as Fusarium and Apiotrichum. Meanwhile, the abundances of beneficial fungi (e.g., Saitozyma and Trichoderma) and bacteria (e.g., Bacillus and Sporosarcina) were significantly decreased. Further investigation demonstrated that root exudate trehalose exhibited prominent bidirectional regulatory effect through promoted the growth of pathogen, while simultaneously inhibiting biofilm formation, rhizosphere colonization at specific concentrations and weakened the biocontrol functions of the beneficial antagonistic bacteria Sporosarcina pasteurii T21 and Lysinibacillus sp. T23, facilitating the formation of a rhizosphere chemical environment that "aids enemies and harms allies" and thereby exacerbating disease occurrence. This study emphasized the dominant role of plant metabolites such as trehalose in driving the assembly of rhizosphere microbial communities from a disease-suppressive to a disease-conducive state, as well as in disease development. The findings provide a novel theoretical perspective for the microbiological regulation of tea root rot and offer theoretical and practical bases for tea root rot disease green prevention and control.