文献类型：期刊文献

中文题名：3味中草药组方提取物的抗菌与抗癌活性研究

英文题名：Study on the antibacterial and anticancer activities of the extract of three Chinese herbs

作者：黄丽荣 王星慧 骆衡

第一作者：黄丽荣

机构：[1]贵州理工学院食品药品制造工程学院;[2]贵州省中国科学院天然产物化学重点实验室

第一机构：贵州理工学院食品药品制造工程学院

年份：2017

卷号：28

期号：12

起止页码：2888-2891

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：中国博士后科学基金特别资助项目(2015T80106);北京市博士后科学基金(2015-ZZ-45);中国科学院"西部青年学者"培养计划项目;贵州省自然科学基金(黔科合J字[2014]2099号);贵州省科学技术基金计划项目(黔科合基础[2016]1069);贵州省科技合作计划项目(黔科合LH字[2016]7092)

语种：中文

中文关键词：金银花;蒲公英;紫花地丁;水提取物;抗菌活性;抗癌活性

外文关键词：Flos Lonicerae ; Dandelion ; Viola yedoensis ; Aqueous extract; Antibacterial activity ; Anticancer activity

摘要：目的研究3味中草药(金银花、蒲公英、紫花地丁)不同组合的水提取液的抗菌和抗癌活性。方法微孔浊度法检测三种药材不同组合的水提取液对金黄色葡萄球菌、枯草芽胞杆菌、大肠杆菌生长的抑制作用;MTT法检测水提取液对白血病细胞K562、前列腺癌细胞PC3、乳腺癌细胞MDA生长的抑制作用,并用流式细胞术及倒置荧光显微镜检测其对癌细胞的诱导凋亡作用。结果不同组合的3味中药水提取液均具有一定的抗菌及抗癌活性;3味中草药同等质量组合的水提取液的抗菌活性和抗白血病细胞K562生长作用最强,且能显著诱导K562细胞凋亡。结论 3味中药等质量组合的水提液的抗菌和抗癌活性最强,抗癌作用具有选择性。
Objective To evaluate the antibacterial and anticancer activities of the aqueous extract of different combinations of three Chinese herbs （Flos Lonicerae, Dandelion, Viola yedoensis ）. Methods The antibacterial activity against three bacteria Escherichia coli, Staphylococcus aureus and Bacillus subtilis of the aqueous extract was determined by turbidimetry assays; the anticancer activity for leukemia cells K562, prostate cancer ceils PC3 and breast cancer cells MDA of the extract was assayed by MTT methods, and activity of cell apoptosis induction was further detected by flow cytometry and fluorescence microscope. Re- sults Different combinations of the aqueous extract could inhibit the growth of both bacteria and cancer ceils in different extent, the best activity of the extract was combined with three herbs in same quantity, which was showed significantly apoptosis induction of K562 cells. Conclusion The aqueous extract of the three herbs combined with equal quantity had potent antibacterial and anti- cancer activity. Moreover, the anticancer activity had selectivity.