文献类型：期刊文献

英文题名：Development of Novel Bacteriostatic Agents Derived from Natural Product Formononetin and Their Action Mechanisms in Crop Diseases Management

作者：Zhang, Miaohe Feng, Shuang Song, Junrong Xue, Wei

第一作者：Zhang, Miaohe;张妙鹤

通信作者：Xue, W[1]

机构：[1]Guizhou Univ, Ctr R&D Fine Chem, State Key Lab Green Pesticide, Guiyang 550025, Peoples R China;[2]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550025, Peoples R China;[3]Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Peoples R China;[4]Nat Prod Res Ctr Guizhou Prov, Guiyang 550014, Peoples R China

第一机构：Guizhou Univ, Ctr R&D Fine Chem, State Key Lab Green Pesticide, Guiyang 550025, Peoples R China

通信机构：corresponding author), Guizhou Univ, Ctr R&D Fine Chem, State Key Lab Green Pesticide, Guiyang 550025, Peoples R China.

年份：2025

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001530839000001)】；

基金：Authors gratefully acknowledge the Science Foundation of Guizhou Province (No. ZK2024008) and Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center (1630042025010).

语种：英文

外文关键词：formononetin; piperidine; antibacterial activity; Xanthomonas oryzae pvoryzae; Xanthomonas axonopodis pv citri; action mechanism

摘要：The present study involved the development of a series of novel antibacterial compounds based on the structure of the natural product formononetin. The in vitro activity experiments demonstrated that compound M34 exhibited the most potent inhibitory effects against Xanthomonas oryzae pvoryzae (Xoo) and Xanthomonas axonopodis pv citri (Xac), with EC50 values of 0.9 and 2.4 mu g/mL, respectively. Pot experiments verified the excellent curative and protective effects of M34 against bacterial leaf blight of rice (BLB) and citrus canker (CC). Furthermore, a series of physiological and biochemical experiments were conducted to investigate the action mechanism exerted by M34 against plant pathogens Xoo and Xac. The findings revealed that M34 effectively inhibited the expression of multiple pathogenic factors, disrupted their energy metabolism, altered their cell membrane morphology, enhanced the activity of host defense enzymes, and attenuated their virulence toward host cells. Therefore, M34 could serve as a promising candidate drug for the inhibition of crop pathogens.