文献类型：期刊文献

英文题名：Signal amplified colorimetric nucleic acid detection based on autocatalytic hairpin assembly

作者：Liu, Yunhua Jin, Limin Mao, Jianfei Deng, Ru Lin, Fengyi Cheng, Yuxin Li, Min Dai, Jianyuan

第一作者：刘云花

通信作者：Liu, YH[1];Dai, JY[2]

机构：[1]Guizhou Inst Technol, Sch Chem Engn, Guiyang 550000, Peoples R China;[2]Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China;[3]Inst Biomed Engn, Chinese Acad Med Sci, Peking Union Med Coll, Tianjin 300192, Peoples R China

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Chem Engn, Guiyang 550000, Peoples R China;corresponding author), Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China.|贵州理工学院化学工程学院;贵州理工学院;

年份：2024

卷号：14

期号：24

起止页码：17152-17157

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20242216180521);Scopus(收录号：2-s2.0-85194428326);WOS:【SCI-EXPANDED(收录号:WOS:001233853200001)】；

基金：This work was supported by the Cooperative Project of Chengdu Xiaojia Technology Co., Ltd (No. 22H1111), Cooperative Project of Analysis and Testing Center of Sichuan Academy of Agricultural Science (No. 20H1090, 0020303410001), the Science and Technology Planning Project of Guizhou Province (No. Qiankehezhicheng [2020]1Y167).

语种：英文

外文关键词：Assembly - Biomolecules - Chemical detection - Color - Colorimetry

摘要：Herein, a nucleic acid assay based on autocatalytic hairpin assembly (ACHA) was proposed. In this system, two split G-quadruplex sequences were integrated into H1 and H2, respectively. And a DNA strand with the same sequence to target DNA was integrated into the assistant hairpin H3. In the presence of target DNA, the hairpin structure of H1 was opened and catalytic hairpin assembly (CHA) was activated, and then a series of DNA assembly steps based on the toehold-mediated DNA strand displacement were triggered and the product H1-H2 with sticky ends on both sides was formed. On the one side of H1-H2, the split two G-quadruplex sequences were close enough to form the intact G-quadruplex for the signal readout. At the same time, two sticky ends on the other side of H1-H2 hybridized with H3 and a new sticky end with the sequence same to the target DNA was exposed, which can immediately trigger the autocatalytic hairpin assembly reaction, and then the reaction rate of CHA was effectively accelerated and the colorimetric signal was significantly amplified. This ACHA signal amplified strategy has been successfully applied for the rapid and colorimetric nucleic acid detection. Herein, a nucleic acid assay based on autocatalytic hairpin assembly (ACHA) was proposed.