文献类型：期刊文献

英文题名：Production of minor ginsenosides by combining Stereum hirsutum and cellulase

作者：Yang, Wenhua Zhou, Jianli Harindintwali, Jean Damascene Yu, Xiaobin

第一作者：Yang, Wenhua

通信作者：Yu, XB[1];Yu, XB[2]

机构：[1]Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi, Jiangsu, Peoples R China;[2]Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi, Jiangsu, Peoples R China;[3]Guizhou Inst Technol, Sch Food & Drug Mfg Engn, Guiyang, Guizhou, Peoples R China

第一机构：Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi, Jiangsu, Peoples R China

通信机构：corresponding author), Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Carbohydrate Chem & Biotechnol, Wuxi, Jiangsu, Peoples R China;corresponding author), Jiangnan Univ, Sch Biotechnol, Key Lab Ind Biotechnol, Minist Educ, Wuxi, Jiangsu, Peoples R China.

年份：2021

卷号：16

期号：8

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000685266400008)】；

基金：This project was funded by the double first class university plan (LITE2018-11) and the Graduate Research and Innovation Projects of Jiangsu Province (SJCX19_0774).

语种：英文

摘要：Minor ginsenosides (MGs) (include ginsenoside F-2, Compound K, PPT, etc), which are generally not produced by ginseng plants naturally, are obtained by deglycosylation of major ginsenosides. However, the conventional processes used to produce deglycosylated ginsenosides focus on the use of intestinal microorganisms for transformation. In this study, an edible and medicinal mushroom Stereum hirsutum JE0512 was screened from 161 beta-glucosidase-producing soil microorganisms sourced from wild ginseng using the plate coloration method. Furthermore, JE0512 was used for the production of CK from ginseng extracts (GE) in solid-state fermentation (SSF) using 20 g corn bran as substrate, 4 g GE, and 20% inoculation volume, and the results showed that the highest CK content was 29.13 mg/g. After combining S. hirsutum JE0512 with cellulase (Aspergillus niger), the MGs (F-2, CK, and PPT) content increased from 1.66 to 130.79 mg/g in the final products. Our results indicate that the Stereum genus has the potential to biotransform GE into CK and the combination of S. hirsutum JE0512 and cellulase could pave the way for the production of MGs from GE.