文献类型：期刊文献

英文题名：Effects of co-overexpression of ALDH1, CYP71AV1, and iaaM on the density, length, and width of glandular secretory trichomes and the contents of artemisinin in Artemisia annua

作者：Zhao, Yan Xiao, Nan Long, Yanxing Luo, Jie Liu, Xiaozhu Zhang, Xuewen

第一作者：Zhao, Yan

通信作者：Zhang, XW[1];Liu, XZ[2]|[14440df22f06bbcaad0e9]刘晓柱;

机构：[1]Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China;[2]Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China

第一机构：Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China

通信机构：corresponding author), Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China;corresponding author), Guizhou Inst Technol, Coll Food & Pharmaceut Engn, Guiyang 550000, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2023

外文期刊名：CANADIAN JOURNAL OF PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001126157400001)】；

基金：Funding information This work was supported by Key Laboratory of Crop Epige-netic Regulation and Development (2019JJ40123) .

语种：英文

外文关键词：Artemisia annua; ALDH1; CYP71AV1; iaaM; artemisinin

摘要：Sesquiterpene lactone artemisinin is one of the main agents used to treat malaria. Artemisinin is produced in the glandular secretory trichomes (GSTs) of Artemisia annua. The plant hormones and metabolic pathways regulate the artemisinin content of A. annua. It was possible to examine the functions of auxin, an important plant hormone, in the development of GSTs in A. annua by enhancing the expression of iaaM, which encodes a tryptophan monooxygenase involved in the biosynthesis of auxin. Additionally, the effects of co-overexpression of aldehyde dehydrogenase 1 (ALDH1), P450 monooxygenase (CYP71AV1), and iaaM on the density, length, and width of GSTs and the contents of artemisinin were further investigated. Results indicated that overexpression of iaaM might increase the density, length, and width of GSTs by enhancing auxin biosynthesis. This study also proved the key regulatory role of ALDH1 in the biosynthesis of artemisinin. Moreover, co-overexpression of ALDH1, CYP71AV1, and iaaM successfully increased the density, length, and width of GSTs and improved the artemisinin content in A. annua. Therefore, we established a theoretical basis for modifying artemisinin accumulation in this study by regulating the expression of auxin and artemisinin synthesis-related genes using a metabolic engineering method.