文献类型：期刊文献

英文题名：Phylogenetic and Morphological Characterization of Spegazzinia Species from Oryza sativa L. in Thailand

作者：Tian, Xing-Guo Han, Jia-Jun Karunarathna, Samantha C. Tibpromma, Saowaluck Lu, Yongzhong Jayawardena, Ruvishika S. Bao, Dan-Feng

第一作者：田兴国;Tian, Xing-Guo

通信作者：Bao, DF[1];Bao, DF[2]

机构：[1]Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550025, Guizhou, Peoples R China;[2]Guizhou Acad Agr Sci, Guizhou Key Lab Agr Microbiol, Guiyang 550009, Peoples R China;[3]Chiang Mai Univ, Fac Agr, Dept Entomol & Plant Pathol, Chiang Mai 50200, Thailand;[4]Qujing Normal Univ, Coll Biol & Food Engn, Ctr Yunnan Plateau Biol Resources Protect & Utiliz, Qujing 655099, Peoples R China;[5]Qujing Normal Univ, Coll Biol & Food Engn, Yunnan Int Joint Lab Fungal Sustainable Utilizat S, Qujing 655099, Peoples R China;[6]Mae Fah Luang Univ, Ctr Excellence Fungal Res, Chiang Rai 57100, Thailand;[7]Guizhou Univ, Engn Res Ctr Utilizat Characterist Biopharmaceut R, Minist Educ, Guiyang 550025, Peoples R China

第一机构：贵州理工学院食品药品制造工程学院

通信机构：corresponding author), Guizhou Inst Technol, Sch Food & Pharmaceut Engn, Guiyang 550025, Guizhou, Peoples R China;corresponding author), Guizhou Univ, Engn Res Ctr Utilizat Characterist Biopharmaceut R, Minist Educ, Guiyang 550025, Peoples R China.|贵州理工学院食品药品制造工程学院;贵州理工学院;

年份：2026

卷号：53

期号：1

外文期刊名：CHIANG MAI JOURNAL OF SCIENCE

收录：;Scopus(收录号：2-s2.0-105028242483);WOS:【SCI-EXPANDED(收录号:WOS:001664242800005)】；

基金：Xing-Guo Tian thanks the Science and Technology Foundation of Guizhou Province (Qian Ke He Pingtai ZSYS [2025] 029) and the Science and Technology Program of Guizhou Province [No. Qian Ke He Jichu QN (2025) 215] . Dan-Feng Bao would like to thank the Postdoctoral Fellowship Program of CPSF under Grant Number GZC20240346 and Guizhou Provincial Basic Research Program (Natural Science) [No. QianKeHe Basic -ZK (2025) general program 666] . SCK, and ST thank the Yunnan Revitalization Talents Support Plan (High-End Foreign Experts Program) and the Key Laboratory of Yunnan Provincial Department of Education of the Deep-Time Evolution on Biodiversity from the Origin of the Pearl River for their support. SCK also thanks the National Natural Science Foundation of China (No. 32260004) for the support.

语种：英文

外文关键词：Didymosphaeriaceae; morphology; new host record; phylogeny; rice-associated microfungi

摘要：Spegazzinia, a genus within the family Didymosphaeriaceae, is known for its pleomorphic conidia and diverse ecological roles. As a vital cereal crop, rice (Oryza sativa L.) supports a rich fungal community, yet many associated fungi lack thorough taxonomic validation combining morphology and molecular data. This study identifies and characterizes Spegazzinia species associated with rice in Thailand by collecting dead rice leaves in Chiang Rai Province. Fungal isolates were obtained through single-spore isolation, and their morphological traits were examined. Phylogenetic analyses were conducted using concatenated sequences from four gene regions, including large subunit nuclear rDNA (LSU), internal transcribed spacer (ITS), small subunit nuclear rDNA (SSU), and translation elongation factor 1-alpha (tef1-alpha). Maximum likelihood and Bayesian inference phylogenetic trees indicated that two isolates clustered with Spegazzinia deightonii and S. tessarthra. Morphological traits aligned with these species, including the production of two conidial types (alpha and beta) with distinctive structural features. The isolate of S. tessarthra represents a new host record for Spegazzinia on rice, broadening its known host spectrum, while S. deightonii, first reported in 1971, is herein recollected on rice in this study. This research emphasizes the significance of integrating morphological and molecular approaches in fungal taxonomy, enhancing our understanding of fungal diversity in rice ecosystems.