文献类型：期刊文献

中文题名：基于G-四链体-氯化血红素DNA酶比色法测定银离子和汞离子传感器的构筑

英文题名：Fabrication of a Sensor for Colorimetric Determination of Silver and Mercury Ions Based on G-quadruplex-Hemin DNAzymes

作者：肖志友 司恒丹 邓兰清 龙丽 居荣梅 张鑫 刘益飞

第一作者：肖志友

机构：[1]贵州理工学院化学工程学院

第一机构：贵州理工学院化学工程学院

年份：2019

卷号：38

期号：7

起止页码：840-844

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金资助项目(21768002);贵州省科学技术基金资助项目(黔科合J字[2013]2263号);贵州理工学院高层次人才科研启动经费项目(XJGC20181213);贵州理工学院大学生创新训练项目(201814440229)

语种：中文

中文关键词：G-四链体-氯化血红素DNA酶;比色法测定;银离子;汞离子;传感器

外文关键词：G-quadruplex-hemin DNAzymes;colorimetric determination;silver ion;mercury ion;sensor

摘要：利用G-四链体DNA(5′-CTGGGAGGGAGGGAGGGA-3′)与氯化血红素结合形成G-四链体-HeminDNA酶,其能高效催化H2O2氧化反应底物由无色变为绿色,当溶液中有Ag^+或Hg^2+存在时会阻碍该DNA酶的形成,导致绿色溶液变浅。基于此,建立了比色法测定Ag^+和Hg^2+的传感器。在最佳实验条件下,溶液的吸光度与Ag^+和Hg^2+浓度分别在100.0~1000.0nmol/L和80.0~800.0nmol/L范围内具有良好的线性关系,检出限(3δ/Slope)分别为55.9nmol/L和64.3nmol/L。该方法具有较好的选择性,采用该方法对实际样品进行测试,结果满意。
A sensor for colorimetric determination of silver and mercury ions based on G-quadruplex-hemin DNAzymes was fabricated.The G-quadruplex-hemin DNAzymes was formed by the combination of G-quadruplex(5′-CTGGGAGGGAGGGAGGGA-3′) with hemin,which could catalyze H2O2 to oxide the substrate efficiently,and make the colorless solution become green.The presence of Ag^+ or Hg^2+ in the solution would hinder the formation of the DNAzyme,and the solution color became lighter.Under the optimal experimental conditions,there were good linear relationships for the absorbance of the solution with Ag^+ and Hg^2+ in the concentration ranges of 100.0-1 000.0 nmol/L and 80.0- 800.0 nmol/L,with detection limits(3 δ/Slope) of 55.9 nmol/L and 64.3 nmol/L,respectively.This method was of good selectivity,and was applied in the detection of real samples with satisfactory results.