文献类型：期刊文献

英文题名：miR-29b-3p inhibits 22Rv1 prostate cancer cell proliferation through the YWHAE/BCL-2 regulatory axis

作者：Zhao, Jiafu Ma, Xiaoyan Xu, Houqiang

第一作者：Zhao, Jiafu

通信作者：Xu, HQ[1]

机构：[1]Guizhou Univ, Key Lab Anim Genet, Breeding & Reprod Plateau Mountainous Reg, Minist Educ, Guiyang 550025, Guizhou, Peoples R China;[2]Guizhou Univ, Coll Anim Sci, Guiyang 550025, Guizhou, Peoples R China;[3]Guizhou Inst Technol, Coll food & pharmaceut Engn, Guiyang, Guizhou, Peoples R China;[4]Guizhou Univ, Coll Anim Sci, 197 Huashi Rd,Huaxi, Guiyang 550025, Guizhou, Peoples R China

第一机构：Guizhou Univ, Key Lab Anim Genet, Breeding & Reprod Plateau Mountainous Reg, Minist Educ, Guiyang 550025, Guizhou, Peoples R China

通信机构：corresponding author), Guizhou Univ, Coll Anim Sci, 197 Huashi Rd,Huaxi, Guiyang 550025, Guizhou, Peoples R China.

年份：2022

卷号：24

期号：2

外文期刊名：ONCOLOGY LETTERS

收录：;Scopus(收录号：2-s2.0-85133915412);WOS:【SCI-EXPANDED(收录号:WOS:000838322200001)】；

基金：The present study was supported by the Natural Science Foundation of Guizhou Province [grant no. QKHJC (2020) 1Y085], the Natural Science Foundation of China (grant no. 31860242) and the Guizhou Province Science and Technology Plan Project [grant no. Guizhou Branch Platform Talents (2019) 3336].

语种：英文

外文关键词：microRNA-29b-3p; metastatic CRPC; YWHAE; cell proliferation; regulatory axis

摘要：Prostate cancer (PCa) is one of the most common malignant tumours in the world and seriously affects health of men. Studies have shown that microRNA (miR)-29b-3p and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon (YWHAE) play important roles in influencing the proliferation and apoptosis of PCa cells. However, the molecular mechanism of miR-29b-3p and YWHAE in the proliferation and apoptosis of PCa cells remains unclear. In the present study, bioinformatics as well as in vivo and in vitro experiments were used to predict and verify the targeting relationship between YWHAE and mir-29B-3p and investigate the potential roles of YWHAE and mir-29b-3p in the proliferation and apoptosis of 22RV1 cells. Using bioinformatics and a double luciferase system assay, it was confirmed that miR-29b-3p can target YWHAE 3 ' untranslated region and affect the expression of YWHAE, suggesting that miR-29b-3p may be a potential miRNA of YWHAE. Reverse transcription-quantitative PCR, Cell Counting Kit-8, Transwell and cell scratch assays showed that miR-29b-3p significantly inhibited the proliferation, invasion and migration of 22Rv1 cells (P<0.01). Rescue experiments demonstrated that YWHAE gene introduction reversed the inhibitory effect of miR-29b-3p on 22Rv1 cells. Western blotting revealed that the upregulation of miR-29b-3p inhibited YWHAE expression, resulting in a very significant decrease in the ratio of p-BAD/BAD and full-length caspase 3/cleaved caspase 3 (P<0.01) and an extremely significant increase in the ratio of BAX/BCL-2 (P<0.01). A tumourigenesis test in nude mice in vivo confirmed that the upregulation of miR-29b-3p inhibited tumour growth by targeting YWHAE. The present experiments confirmed that miR-29b-3p plays a tumour suppressor role in 22Rv1 PCa cells, and the YWHAE/BCL-2 regulatory axis plays a vital role in miR-29b-3p regulating the proliferation and apoptosis of 22Rv1 cells. These results may provide a theoretical basis for the diagnosis and targeted treatment of PCa.