文献类型：期刊文献

英文题名：An In Vitro Model of Biomimetic Vascular Cell Spatial Arrangement Based on Fiber-Reinforced Biocomposite

作者：Dai, Shuli Tong, Kun Duan, Xiaofeng Lu, Danting Guo, Rui Zou, Zhongfei Xie, Zhenwei Zhao, Li Li, Jiachun Chen, Yuewei

第一作者：Dai, Shuli

通信作者：Duan, XF[1];Li, JC[2];Chen, YW[2];Duan, XF[3]

机构：[1]Guizhou Univ, Med Coll, Guiyang 550025, Guizhou, Peoples R China;[2]Guizhou Univ, Mech Engn Coll, Guiyang 550025, Guizhou, Peoples R China;[3]Guizhou Prov Peoples Hosp Oral & Maxillofacial Sur, Guiyang 550002, Guizhou, Peoples R China;[4]Guizhou Univ, North Alabama Int Collage Engn & Technol, Guiyang 550025, Guizhou, Peoples R China;[5]Guizhou Inst Technol, Sch Mech Engn, Guiyang 550003, Guizhou, Peoples R China;[6]Zhejiang Univ, Womens Hosp, Sch Med, Dept Gynecol, Hangzhou 310026, Zhejiang, Peoples R China

第一机构：Guizhou Univ, Med Coll, Guiyang 550025, Guizhou, Peoples R China

通信机构：corresponding author), Guizhou Univ, Med Coll, Guiyang 550025, Guizhou, Peoples R China;corresponding author), Guizhou Univ, Mech Engn Coll, Guiyang 550025, Guizhou, Peoples R China;corresponding author), Guizhou Prov Peoples Hosp Oral & Maxillofacial Sur, Guiyang 550002, Guizhou, Peoples R China.

年份：2026

外文期刊名：ACS APPLIED POLYMER MATERIALS

收录：;EI(收录号：20261620512983);Scopus(收录号：2-s2.0-105035704887);WOS:【SCI-EXPANDED(收录号:WOS:001722448000001)】；

基金：This work was supported by the Science and Technology Planning Project of Guizhou Province (grant nos. MS [2025]615, ZK [2024]510), the National Natural Science Foundation of China (grant nos. 52565030, 52565008, 52465035), the National Health Commission Scientific Research Fund-Zhejiang Provincial Major Science and Technology Program for Health (WKJ-ZJ-2507), and the Introduced Talents Research Project of Guizhou University (GDRJHZ-202416).

语种：英文

外文关键词：bionic vascular models; drug screening; spatialarrangement; gelatin-based hydrogel; bilayer vasculartissue model; circumferential direction

摘要：In vitro construction of bionic vascular models plays a pivotal role in the research of vascular diseases and drug screening. The existing vascular models in vitro have limitations in terms of the insufficient spatial arrangement of vascular cells to achieve functionalization. In this study, a cell adhesion-scaffold composite system assisted by degradable gelatin-based hydrogel was proposed to realize the implantation of vascular cells in the tubular space structure. A bilayer vascular tissue model was constructed in vitro, in which Smooth muscle cells (SMCs) were arranged in a circumferential direction, and Endothelial cells (ECs) were uniformly and continuously distributed along the inner wall of the SMC. Melt electrowriting was used to print a polycaprolactone high-precision tubular scaffold, which provided mechanical support while effectively guiding the circumferential arrangement of SMCs and high-density spatial planting. By comparing SMCs cultured on two-dimensional (2D) disoriented fibers, three-dimensional (3D) disoriented fibers, and 3D circumferential fibers, the expression of alpha-smooth muscle actin (alpha-SMA) was significantly enhanced in cells cultured on 3D oriented fibers, and the expression of contraction-related genes increased by about 2.5 times compared with those cultured on 2D disoriented fibers. ECs were seeded onto the inner surface of the SMC layer, resulting in the spatial construction of the two vascular cell types. The bilayer vascular tissue cell model constructed by this method provides an approach for the screening and evaluation of vascular-related drugs in vitro.