文献类型：期刊文献

英文题名：Functional Restoration of Uterine Architecture and Fertility via a 3D-Printed Biomimetic Scaffold: The Role of Macrophage-Driven Immunomodulation and YAP Signaling

作者：Wei, Qianqian Zhang, Jing Weng, Jiahao Lan, Jiawen He, Jing Chen, Yuewei Zou, Zhongfei He, Yong Wang, Yanpeng Shu, Jing

第一作者：Wei, Qianqian

机构：[1] Center for Reproductive Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang, Hangzhou, China; [2] State Key Laboratory of Fluid Power and Mechatronic Systems, Liangzhu Laboratory, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310027, China; [3] Center for Reproductive Medicine, Department of Gynecology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Zhejiang, Hangzhou, China; [4] School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College, Zhejiang, Hangzhou, China; [5] The Second Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, 310027, China; [6] Zhejiang Key Laboratory of Additive Manufacturing Technology and Equipment, College of Mechanical Engineering, Zhejiang University, Hangzhou, 310027, China; [7] School of Mechanical Engineering, Guizhou University, Guiyang, 550025, China; [8] School of Mechanical Engineering, Guizhou Institute of Technology, Guiyang, 550003, China; [9] Zhejiang University, China

第一机构：Center for Reproductive Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Zhejiang, Hangzhou, China

年份：2025

外文期刊名：SSRN

收录：EI(收录号：20250538643)

语种：英文

外文关键词：3D reconstruction - Biomechanics - Biomimetics - Cell culture - Cell proliferation - Cell signaling - Image reconstruction - Macroinvertebrates - Macrophages - Polarization - Reconstruction (structural) - Regenerative medicine - Restoration - Scaffolds (biology) - Signaling - Tissue - Tissue regeneration

摘要：The structural complexity of the uterus presents a significant challenge in identifying biomaterials and scaffold designs capable of supporting functional tissue regeneration. Poly(L-lactide-co-caprolactone) (PLCL) has attracted attention due to its biocompatibility and a degradation profile that can be modulated. In this study, a three-layered PLCL scaffold was fabricated via melt electrowriting (MEW) 3D printing to mimic the mechanical properties of the native uterine matrix. Using a mouse model with severe uterine injury, we demonstrated that the PLCL scaffold markedly promoted structural repair and functional recovery, as evidenced by improved pregnancy outcomes. Mechanistic insights revealed that the scaffold enhanced macrophage recruitment and skewed polarization toward an M2 phenotype, accompanied by upregulation of hallmark genes SPP1, LGALS3, and TREM2. Furthermore, the scaffold facilitated parenchymal cell proliferation and migration and activated the JAK-STAT3 and YAP-Hippo signaling pathways, thereby orchestrating a pro-regenerative niche. Collectively, these findings elucidate a scaffold-driven immunomodulatory mechanism that promotes uterine regeneration through macrophage polarization and key signaling activation. ? 2025, The Authors. All rights reserved.