文献类型：期刊文献

英文题名：Chitosan-based cross-linked fluorescent polymer containing aggregation-induced emission fluorogen for cell imaging

作者：Xie, Gaoyi Ma, Chunping Zhang, Xiqi Liu, Hongliang Yang, Liutao Li, Yang Wang, Ke Wei, Yen

第一作者：谢高艺

通信作者：Ma, CP[1];Zhang, XQ[2];Wei, Y[3];Wei, Y[4]|[144404c59386af0196f94]马春平;

机构：[1]Guizhou Inst Technol, Coll Chem Engn, Guiyang 550003, Peoples R China;[2]Guizhou Inst Technol, Coll Mat & Met Engn, Guiyang 550003, Peoples R China;[3]Chinese Acad Sci, Tech Inst Phys & Chem, CAS Key Lab Bioinspired Mat & Interface Sci, Beijing 100190, Peoples R China;[4]Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China;[5]Tsinghua Univ, Tsinghua Ctr Frontier Polymer Res, Beijing 100084, Peoples R China;[6]Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China

第一机构：贵州理工学院化学工程学院

通信机构：corresponding author), Guizhou Inst Technol, Coll Mat & Met Engn, Guiyang 550003, Peoples R China;corresponding author), Chinese Acad Sci, Tech Inst Phys & Chem, CAS Key Lab Bioinspired Mat & Interface Sci, Beijing 100190, Peoples R China;corresponding author), Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China;corresponding author), Tsinghua Univ, Tsinghua Ctr Frontier Polymer Res, Beijing 100084, Peoples R China.|贵州理工学院材料与冶金工程学院;贵州理工学院;

年份：2017

卷号：143

起止页码：276-283

外文期刊名：DYES AND PIGMENTS

收录：;EI(收录号：20171803627266);Scopus(收录号：2-s2.0-85018349042);WOS:【SCI-EXPANDED(收录号:WOS:000403122900034)】；

基金：This research was supported by the National Science Foundation of China (Nos. 51603211, 51503046, 51673107, 21604048), the MOST of China (2016YFA200800), the Guizhou Province Science and Technology Planning Project ([2015]2068), and the Scientific&Technological Innovation Talent Team of Guizhou Province (Qian Ke He Talent Team [2015]4008).

语种：英文

外文关键词：Aggregation-induced emission; Cross-linked fluorescent polymer; Chitosan; Particle stability; Photostability; Biocompatibility

摘要：A chitosan-based cross-linked fluorescent polymer (Ch-EP3) containing aggregation-induced emission (AIE) fluorogen was prepared through ring-opening polymerization of carboxymethyl chitosan and an AIE monomer with two ethylene oxide groups. H-1 NMR, FT-IR, and X-ray photoelectron spectra were conducted to characterize and prove its successful synthesis. The introduction of chitosan component endowed Ch-EP3 with amphiphilic property, and Ch-EP3 could assemble into nanoparticles, which were facilely dispersed in water to form fluorescent polymeric nanoparticles (FPNs). The dynamic light scattering and transmission electron microscopy results indicated the size distribution of the FPNs was 260 +/- 12 nm with spherical shape. Fluorescent spectrum of the prepared FPNs demonstrated intense green fluorescence with high fluorescence quantum yield of 35%. The Ch-EP3 FPNs further showed excellent particle stability at highly dilute solution with low critical micelle concentration of 0.063 mg mL(-1). The synthesized FPNs also indicated high photostability under UV light irradiation for a period of 60 min. The cytotoxicity evaluation proved high cell viability after incubation of the Ch-EP3 FPNs at different concentrations for a period of 8 h or 24 h, and demonstrated highly biocompatible for cell imaging. Taking advantage of the high-performance Ch-EP3 FPNs, such as, high water dispersibility, good particle stability, and excellent photostability, this work would inspire more approaches to prepare novel cross-linked biocompatible fluorescent polymers for biomedical application. (C) 2017 Elsevier Ltd. All rights reserved.