文献类型：期刊文献

中文题名：一测多评法测定响铃草中槲皮素和山柰素含量

英文题名：Determination of Quercetin and Kaempferol in Crotalaria ferruginea by QAMS

作者：李燕 吴德智 姜光辉 赵波燕

第一作者：李燕

机构：[1]贵州理工学院

第一机构：贵州理工学院

年份：2018

卷号：33

期号：5

起止页码：538-542

中文期刊名：福建农业学报

外文期刊名：Fujian Journal of Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2017】；

基金：贵州省科学技术基金计划(黔科合LH字[2016]7089)

语种：中文

中文关键词：响铃草;槲皮素;山柰素;一测多评法;高效液相色谱;相对校正因子

外文关键词：Crotalaria ferruginea;quercetin;kaempferol;QAMS;HPLC;relative correction factors

摘要：为建立适用于响铃草质量控制的槲皮素和山柰素含量的一测多评测定方法,采用高效液相色谱HPLC法,使用C18色谱柱(4.6mm×250mm,5μm),乙腈∶0.4%醋酸(40∶60)为流动相,检测波长360nm,柱温30℃,流速1.0mL·min-1,检测槲皮素和山柰素含量。以槲皮素为内标物,测定其与山柰素的相对校正因子(RCF),并进行含量计算,实现一测多评;同时采用外标法(ESM)测定响铃草中2种有效成分的含量,将一测多评法(QAMS)与外标法(ESM)进行比较。结果表明,8批响铃草药材中山柰素含量的一测多评法测定结果均与外标法无显著性差异。说明该方法操作简便,快速,重复性好,结果准确可靠,可为进一步完善响铃草的质量标准提供参考。
To establish a QAMS methodology for simultaneous determination of quercetin and kaempferol contents in Crotalaria ferruginea,HPLC was applied on a C18 column（4.6 mm×250 mm,5μm）with acetonitrile：0.4%CH3 COOH（40∶60）for the mobile phase.The column temperature was kept at 30℃ and flow rate at 1.0 mL·min^-1.The eluate was detected at the wavelength of 360 nm.Quercetin was used as the internal standard to establish the relative correction factor（RCF）for the quantitative analysis of multi-components on QAMS.The RCF of kaempferol was calculated.The contents of the two compounds were measured by both the external standard method and QAMS to verify the validity of the proposed QAMS methodology.The resulting RCF showed good durability on both quercetin and kaempferol determinations.There were no significant differences between the data obtained by QAMS and by the external standard method.Consequently,this new approach was considered simple,rapid and applicable for quality control on raw material and processed products involving C.ferruginea.