文献类型：期刊文献

英文题名：3D printed grafts with gradient structures for organized vascular regeneration

作者：Chen, Yuewei Zou, Zhongfei Fu, Tao Li, Zhuang Zhang, Zhaojie Zhu, Meng Gao, Qing Wu, Shaofei Fu, Guosheng He, Yong Fu, Jiayin

第一作者：Chen, Yuewei

通信作者：He, Y[1];Fu, GS[2];Fu, JY[2]

机构：[1]Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;[2]Guizhou Univ, Sch Mech Engn, Guiyang 550025, Peoples R China;[3]Zhejiang Univ, Sir Run Run Shaw Hosp, Hangzhou, Peoples R China;[4]Guizhou Inst Technol, Sch Mech Engn, Guiyang, Peoples R China;[5]Zhejiang Univ, Sch Med, Sch Stomatol, Sch Med,Affiliated Hosp 2, Hangzhou 310006, Zhejiang, Peoples R China;[6]Key Lab Oral Biomed Res Zhejiang Prov, Hangzhou 310006, Zhejiang, Peoples R China

第一机构：Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China

通信机构：corresponding author), Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou 310027, Peoples R China;corresponding author), Zhejiang Univ, Sir Run Run Shaw Hosp, Hangzhou, Peoples R China.

年份：2024

卷号：6

期号：3

外文期刊名：INTERNATIONAL JOURNAL OF EXTREME MANUFACTURING

收录：;EI(收录号：20241215775941);Scopus(收录号：2-s2.0-85187954214);WOS:【SCI-EXPANDED(收录号:WOS:001185796600001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (Nos. 52235007, 52325504, T2121004) and Zhejiang Province Natural Science Foundation of China under Grant No. LQ23H090012, LQ22H180001 and the Science and Technology of Medicine and Health program of Zhejiang Province (No. 2023RC028).

语种：英文

外文关键词：small-diameter vascular graft; smooth muscle layer; 3D printing; endothelialization

摘要：Synthetic vascular grafts suitable for small-diameter arteries (<6 mm) are in great need. However, there are still no commercially available small-diameter vascular grafts (SDVGs) in clinical practice due to thrombosis and stenosis after in vivo implantation. When designing SDVGs, many studies emphasized reendothelization but ignored the importance of reconstruction of the smooth muscle layer (SML). To facilitate rapid SML regeneration, a high-resolution 3D printing method was used to create a novel bilayer SDVG with structures and mechanical properties mimicking natural arteries. Bioinspired by the collagen alignment of SML, the inner layer of the grafts had larger pore sizes and high porosity to accelerate the infiltration of cells and their circumferential alignment, which could facilitate SML reconstruction for compliance restoration and spontaneous endothelialization. The outer layer was designed to induce fibroblast recruitment by low porosity and minor pore size and provide SDVG with sufficient mechanical strength. One month after implantation, the arteries regenerated by 3D-printed grafts exhibited better pulsatility than electrospun grafts, with a compliance (8.9%) approaching that of natural arteries (11.36%) and significantly higher than that of electrospun ones (1.9%). The 3D-printed vascular demonstrated a three-layer structure more closely resembling natural arteries while electrospun grafts showed incomplete endothelium and immature SML. Our study shows the importance of SML reconstruction during vascular graft regeneration and provides an effective strategy to reconstruct blood vessels through 3D-printed structures rapidly.