文献类型：期刊文献

英文题名：Fluorescent Determination of Calcium Ion by DNA Nanospheres Based Upon the EtNa DNAzyme

作者：Lin, Fengyi Cheng, Yuxin Li, Min Mao, Jianfei Liu, Yunhua Zhou, Cuisong Dai, Jianyuan Guo, Yong Xiao, Dan

第一作者：Lin, Fengyi

通信作者：Dai, JY[1]

机构：[1]Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China;[2]Guizhou Inst Technol, Sch Chem Engn, Guiyang, Peoples R China;[3]Sichuan Univ, Coll Chem Engn, Chengdu, Peoples R China

第一机构：Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China

通信机构：corresponding author), Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China.

年份：2023

外文期刊名：ANALYTICAL LETTERS

收录：;Scopus(收录号：2-s2.0-85180260168);WOS:【SCI-EXPANDED(收录号:WOS:001128799500001)】；

基金：This work was supported by the Cooperative Project of Chengdu Xiaojia Technology (No. 22H1111), Cooperative Project of Analysis and Testing Center of Sichuan Academy of Agricultural Science (No. 20H1090, 0020303410001), the Science and Technology Planning Project of Guizhou Province (No. Qiankehezhicheng (Li, Yongxin et al.)1Y167), and the National Natural Science Foundation of China (No. 21974091).

语种：英文

外文关键词：Biosensor; calcium ion; DNA nanosphere; EtNa DNAzyme; fluorescence

摘要：A Ca2+ sensor based on DNA nanospheres was developed. The DNA nanospheres were obtained by complementary pairing of Y-shaped DNA and DNAzyme, in which Y-shaped DNA is a triple-branched DNA with three sticky ends formed by three partially complementary single-stranded DNAs. DNAzyme is a recently reported RNA-cleaving DNAzyme, EtNa, that has high selectivity for Ca2+ and was used as linker in DNA nanosphere preparation. When EtNa DNAzyme was formed by enzyme chain (E) and substrate chain (S), the fluorescence signal was quenched significantly due to the proximity of fluorophore and quencher. In the presence of Ca2+, it bound to the E strand of EtNa DNAzyme, catalyzing the cleavage of S strand, which led to the separation of S from E and then the DNA nanosphere was disintegrated. At the same time, the fluorescence signal was restored and Ca2+ assay was realized. This detection system based on DNA nanospheres determined Ca2+ across a linear range from 20 to 400 mu M with a detection limit of 1.6 mu M and excellent selectivity. In addition, this proposed Ca2+ assay has been successfully applied to the assay of Ca2+ in human serum, thus demonstrating clinical application.